Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy
Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy
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DOI：
10.1021/ja108404p
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发表时间：
2011-04-27
影响因子：
15
通讯作者：
Sodeoka, Mikiko
中科院分区：
文献类型：
--
作者：
Yamakoshi, Hiroyuki;Dodo, Kosuke;Sodeoka, Mikiko
利用拉曼显微镜首次实现了炔标记细胞增殖探针EdU在活细胞中的核定位无点击成像。炔标签在不受内源分子干扰的细胞拉曼沉默区显示出强烈的拉曼带。这种方法可以在检测炔标记分子时消除点击反应的需要。
Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.