THE TRANSPARENT CHAMBER TECHNIQUE FOR THE MICROSCOPIC STUDY OF LIVING BLOOD VESSELS
THE TRANSPARENT CHAMBER TECHNIQUE FOR THE MICROSCOPIC STUDY OF LIVING BLOOD VESSELS
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DOI：
10.1002/ar.1091200112
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发表时间：
1954-01-01
影响因子：
--
通讯作者：
CLARK, ER
中科院分区：
文献类型：
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作者：
CLARK, ER
在讨论透明小室技术之前，应该先为它的祖先说一句话，那就是研究两栖类幼虫尾巴中的活血管的方法。虽然这种研究方法已经有10多年的历史，在19世纪30年代和19世纪30年代被Schwann和Kiilliker使用，但在世纪之交不久，由于JJ Abell发现了一种新的麻醉剂，即氯酮，一种稳定的氯仿，它又被复兴了。1907年，在约翰·霍普金斯大学医学院的富兰克林·P·莫尔的实验室里，罗斯·G·哈里森(2008年)在个别蝌蚪身上观察了数天神经再生的方式。第二年春天，也是在Mall博士的实验室里，在他的启发下，我开始了对蝌蚪尾巴的研究，使用了一个竖直的小室和新的麻醉剂氯酮。观察结果如下(尔克拉克‘09和’12)。显微镜被放在与试管水平的支架上，与坐着的观察者的眼睛水平。在1比3000，1比5000的氯酮溶液中麻醉的蝌蚪，被放置在一个小的微型水族馆里，这个水族馆建在一个2英寸乘3英寸的玻璃幻灯片上，从前面到后面有大约5个镍的空间，4个ERN长，2个ERN高。那只小蝌蚪一动不动，靠着水族箱底部的一条狭长的盖子玻璃，轻轻地压在尾巴上，与形成水族箱前面的盖子玻璃或云母接触。莱茨特辑《画图眼镜片》
Before taking up the matter of the transparent chamber technique a word should be said for its immediate ancestor, namely, the method of studying the living vessels in the tails of amphibian larvae. While this method of study is more than 10.0 years old, having been used by Schwann and Kiilliker in the 1830s and’~ OS, it was revived just a little after the turn of the century as a result of the discovery, by JJ Abell, of a new form of anesthetic, namely chloretone, a stable form of chloraform. In the laboratory of Franklin P. Mall, ait the Johns Hopkins University School of Medicine, Ross G. Harrison (’08) in 1907 watched, for several days in individual tadpoles, the manner of nerve regeneration. In the spring of the following year, also in Dr. Mall’s laboratory and at his inspiration, I started studies on the tadpole’s tail, using a vertical chamber and the new anesthetic, chloretone. Observations were made as follows.(ER Clark’09 and’12). The microscope was placed on a stand with the tube horizontal, at the eye level of the seated observer. The tadpole, anesthetized in 1 to 3000, to 1 to 5000 chloretone, was placed, still in the chloretone solution, in a small micro-aquarium built up on a 2 inch by 3 inch glass slide containing a space about 5 niin from front to back, 4 ern long and 2 ern high. The tadpole was kelk motionless and in contact with the cover-glass or mica forming the front of the aquarium, by a narrow sliver of cover-glass which rested on the bottom of the aquarium and was pressed lightly against the tail. The Leitz special “drawing eye-piece”