THE ROLES OF PROTEIN-KINASE-C AND INTRACELLULAR CA2+ IN THE SECRETION OF VONWILLEBRAND-FACTOR FROM HUMAN VASCULAR ENDOTHELIAL-CELLS
THE ROLES OF PROTEIN-KINASE-C AND INTRACELLULAR CA2+ IN THE SECRETION OF VONWILLEBRAND-FACTOR FROM HUMAN VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1042/bj2860631
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发表时间：
1992-09-01
影响因子：
4.1
通讯作者：
PEARSON, JD
中科院分区：
文献类型：
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作者：
CAREW, MA;PALEOLOG, EM;PEARSON, JD
本文研究了血管性血友病因子（vWf）糖蛋白从人脐静脉内皮细胞储存颗粒中的分泌。无论是升高细胞内Ca 2+浓度（[Ca 2 +]i）与Ca 2+离子载体或激活蛋白激酶（PK）C的佛波醇12-肉豆蔻酸酯13-乙酸酯引起的vWf分泌，并在一起的代理协同作用。然而，当vWf释放刺激受体介导的激动剂，PKC的选择性抑制组胺诱导的分泌和凝血酶诱导的分泌显着升高没有影响。此外，ATP，有效地提高[Ca 2 +]i在这些细胞中，是一个非常差的vWf释放效应。我们的结论是，升高[Ca 2 +]i的生理激动剂是必要的vWf释放，但其他信号传导机制，尚未表征，但不是由于PKC激活，需要充分诱导的分泌途径。
Secretion of von Willebrand factor (vWf) glycoprotein from storage granules in human umbilical-vein endothelial cells was studied in vitro. Either elevation of intracellular Ca2+ concentration ([Ca2+]i) with a Ca2+ ionophore or activation of protein kinase (PK) C by phorbol 12-myristate 13-acetate caused vWf secretion, and together the agents acted synergistically. However, when vWf release was stimulated by receptor-mediated agonists, selective inhibition of PKC had no effect on histamine-induced secretion and significantly elevated thrombin-induced secretion. Furthermore, ATP, which efficiently elevates [Ca2+]i in these cells, was a very poor effector of vWf release. We conclude that elevation of [Ca2+]i by physiological agonists is necessary for vWf release, but other signalling mechanisms, as yet uncharacterized, but not due to PKC activation, are required for full induction of the secretory pathway.