Evaluation of the oxygen delivery ability of PEG-hemoglobin in Sprague-Dawley rats during hemodilution
Evaluation of the oxygen delivery ability of PEG-hemoglobin in Sprague-Dawley rats during hemodilution
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DOI：
10.3109/10731199809119778
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Shorr, RGL
中科院分区：
文献类型：
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作者：
Conover, C;Linberg, R;Shorr, RGL
聚乙二醇(PEG)的偶联使牛血红蛋白(Hb)在增加其血浆扩张能力的同时保持其氧气输送能力。为了确定PEG-Hb维持生命的能力是否是由于其氧气输送能力，而不是其血浆扩张能力，Spraogue-Dawley大鼠接受了交换输血，以使红细胞压积降低85%，其中包括聚乙二醇Hb、聚乙二醇50%高铁血红蛋白(PEGMHb)、聚乙二醇一氧化碳血红蛋白(PEGCOHb)或聚乙二醇人血清白蛋白(PEGHSA)。在换血过程中、术后5分钟、24小时和48小时监测存活率和呼吸频率。对存活14天的大鼠进行血液学、血液化学和组织病理学检查。注射聚乙二醇化血红蛋白的大鼠在输血过程中存活率为100%，24小时存活率为79%，而聚乙二醇化高铁血红蛋白和聚乙二醇化血红蛋白的24小时存活率分别为30%和0%。在2周观察期内存活的经聚乙二醇化血红蛋白处理的大鼠的血液学和血液化学水平正常，没有明显的形态学影响。因此，这项研究表明，聚乙二醇-HB可以维持生命，而类似的血浆扩张剂的氧气输送能力较差，效果不佳。
Polyethylene glycol (PEG) conjugation allows bovine hemoglobin (Hb) to retain its oxygen delivery capability while increasing its plasma expansion capacity. To determine whether PEG-Hb's ability to sustain life is due to its oxygen delivery capability rather than its plasma expansion capacity, Sprague-Dawley rats were exchange-transfused up to an 85 % hematocrit reduction with either PEG-Hb, PEG-50%-methemoglobin (PEG-mHb), PEG-carbon monoxide hemoglobin (PEG-COHb) or PEG-human serum albumin (PEG-HSA). Survival and respiratory rates were monitored during the exchange transfusion, at five minutes, 24 hours and 48 hours post operative. Rats surviving 14 days were evaluated for hematology, blood chemistry and histopathology. Rats infused with PEG-Hb had a survival rate of 100% during the transfusion and 79% at 24 hours, as compared to 24 hour survival rates of 30% for PEG-mHb, and 0% for both PEG-COHb and PEG-HSA. PEG-Hb treated rats that survived the 2 week observation period had normal hematological and blood chemistry levels and no significant morphological effects. Therefore, this study demonstrates that PEG-Hb can sustain life while similar plasma expansion agents with less oxygen delivery capability are not as effective.