Antitumor activity of common antibiotics against superficial bladder cancer
Antitumor activity of common antibiotics against superficial bladder cancer
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DOI：
10.1016/j.urology.2003.10.038
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发表时间：
2004-03-01
期刊：
影响因子：
2.1
通讯作者：
Lamm, DL
中科院分区：
文献类型：
--
作者：
Kamat, AM;Lamm, DL
Objectives. The recurrence rate for superficial bladder tumors treated with complete resection averages 88%. Intravesical chemotherapy decreases the recurrence rate by only 14%; thus, new chemotherapeutic agents are needed. Antibiotics are often used to prevent infections after transurethral resection of bladder tumors. Oral intake of antibiotics results in significantly greater concentrations in the urine than in the serum. Our objective was to evaluate four commonly used urinary antibiotics for their cytotoxic activity against bladder cancer cells at clinically relevant concentrations.Methods. Three human transitional cell carcinoma lines-HTB9 (grade 2), T24 (grade 3), and TccSup (grade 4)-were exposed to ciprofloxacin, trimethoprim-sulfamethoxazole, cefazolin, or nitrofurantoin at concentrations from 0 (control) to 1000, 1000, 5000, and 2000 mug/mL, respectively, for 96 hours. Cytotoxicity was evaluated using the MTT colorimetric assay. Six replicates were used for each data point, and the results are reported as the mean +/- standard deviation.Results. Significant cytotoxicity [P
