First Report of Potato spindle tuber viroid in Tomato in Belgium.
First Report of Potato spindle tuber viroid in Tomato in Belgium.
复制标题
比利时首次报道番茄中马铃薯纺锤块茎类病毒。
DOI：
10.1094/pdis-91-8-1055a
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发表时间：
2007
期刊：
影响因子：
4.5
通讯作者：
D. Michelante
中科院分区：
文献类型：
--
作者：
J. Verhoeven;C. Jansen;J. Roenhorst;S. Steyer;D. Michelante
在2006年8月期间，从比利时的温室接收番茄植物（Solanum lycopersicum，以前的番茄）的样品用于诊断。植株表现出严重的生长减缓，幼叶失绿和扭曲。在温室里，病害一直在沿着行缓慢蔓延。这些观察结果表明存在类病毒感染，使用两组通用类聚痘病毒引物（Posi 1-RE/FW和Vid-FW/RE; 3）的逆转录酶（RT）-PCR产生了预期大小的扩增子（约196和360 bp）。对较大PCR产物的序列分析显示，该基因组为358 nt，与先前提交给NCBI GenBank的马铃薯纺锤块茎类病毒（PSTVd）的两个分离物（登录号：来自英国的AJ 583449和来自澳大利亚的AY 962324）。因此，与有症状的番茄植物相关的病原体被鉴定为PSTVd。追踪感染的起源揭示了以下信息：在2005年11月期间，由荷兰苗圃从种子培育的8天大的番茄幼苗被转移到比利时种植者的温室的一小部分; 7至8周后，植物被移植到其最终目的地;在2006年5月期间，种植者首次观察到单个植物的生长减少;几周后，在靠近第一个有症状的植株的同一行中的另外两个植株中观察到类似的症状;到9月，大约有20个有症状的番茄植株，都位于相邻的两行中。通过摧毁受影响行以及两侧两个相邻行中的所有番茄植株，完全根除了类病毒爆发。通过RT-PCR（2）和实时RT-PCR（1）检测合并样品中约1，200株番茄植株的PSTVd，证实没有进一步感染。类病毒的起源以及引入和传播的方法仍不清楚。参考文献：（1）N. Boonham等人，J. Virol. Methods 116：139，2004. (2)R. A. Mumford等，Plant Pathol. 53：242，2004. (3)J. Th. J. Verhoeven等人，Eur. J. Plant Pathol. 110：823，2004.
During August of 2006, a sample of a tomato plant (Solanum lycopersicum, formerly Lycopersicum esculentum) from a greenhouse in Belgium was received for diagnosis. The plant showed severe growth reduction and the young leaves were chlorotic and distorted. In the greenhouse, the disease had been spreading slowly along the row. These observations suggested the presence of a viroid infection, and reverse transcriptase (RT)-PCR with two sets of universal pospiviroid primers (Pospi1-RE/FW and Vid-FW/RE; 3) yielded amplicons of the expected size (approximately 196 and 360 bp). Sequence analysis of the larger PCR product revealed that the genome was 358 nt and 100% identical to two isolates of Potato spindle tuber viroid (PSTVd) previously submitted to the NCBI GenBank (Accession Nos. AJ583449 from the United Kingdom and AY962324 from Australia). A pathogen associated with the symptomatic tomato plants was therefore identified as PSTVd. Tracing the origin of the infection revealed the following information: during November of 2005, 8-day-old tomato seedlings raised from seed by a Dutch nursery were transferred to a small part of the greenhouse of the Belgian grower; 7 to 8 weeks later, the plants were transplanted to their final destination; during May of 2006, the grower first observed growth reduction in a single plant; several weeks later, similar symptoms were observed in two more plants in the same row close to the first symptomatic plant; and by September, there were approximately 20 symptomatic tomato plants, all located in two adjacent rows. The viroid outbreak was fully eradicated by destroying all tomato plants in the affected rows as well as in two adjacent rows at both sides. The absence of further infections was confirmed by testing approximately 1,200 tomato plants in pooled samples for PSTVd by RT-PCR (2) and real-time RT-PCR (1). The origin and the method of introduction and spread of the viroid remain unclear. References: (1) N. Boonham et al. J. Virol. Methods 116:139, 2004. (2) R. A. Mumford et al. Plant Pathol. 53:242, 2004. (3) J. Th. J. Verhoeven et al. Eur. J. Plant Pathol. 110:823, 2004.