Whole-genome sequencing of multiple Arabidopsis thaliana populations
Whole-genome sequencing of multiple Arabidopsis thaliana populations
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DOI：
10.1038/ng.911
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发表时间：
2011-10-01
期刊：
影响因子：
30.8
通讯作者：
Weigel, Detlef
中科院分区：
文献类型：
--
作者：
Cao, Jun;Schneeberger, Korbinian;Weigel, Detlef
植物拟南芥自然发生在许多不同的栖息地在整个欧亚大陆。作为识别有助于适应不同环境的遗传变异的基础，1001基因组计划对地理上不同的A。thaliana菌株已经启动。在这里，我们提出了这个项目的第一阶段，根据人口规模的测序80株来自整个物种的原生范围的8个地区。我们描述了大多数常见的小规模多态性，以及许多较大的插入和缺失在A。thaliana泛基因组，它们对基因功能的影响，以及这些变体之间的局部和全局连锁模式。通过比较自A以来积累的突变谱，可以确定自发突变以外的过程的作用。根据实验室观测到的光谱，一千万年前，thaliana与它最近的亲戚分道扬镳。最近物种范围内的选择性扫描是罕见的，潜在的有害突变在边缘人群中更常见。
The plant Arabidopsis thaliana occurs naturally in many different habitats throughout Eurasia. As a foundation for identifying genetic variation contributing to adaptation to diverse environments, a 1001 Genomes Project to sequence geographically diverse A. thaliana strains has been initiated. Here we present the first phase of this project, based on population-scale sequencing of 80 strains drawn from eight regions throughout the species' native range. We describe the majority of common small-scale polymorphisms as well as many larger insertions and deletions in the A. thaliana pan-genome, their effects on gene function, and the patterns of local and global linkage among these variants. The action of processes other than spontaneous mutation is identified by comparing the spectrum of mutations that have accumulated since A. thaliana diverged from its closest relative 10 million years ago with the spectrum observed in the laboratory. Recent species-wide selective sweeps are rare, and potentially deleterious mutations are more common in marginal populations.