Nucleocapsid of Tomato spotted wilt tospovirus forms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K
Nucleocapsid of Tomato spotted wilt tospovirus forms mobile particles that traffic on an actin/endoplasmic reticulum network driven by myosin XI-K
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番茄斑枯病病毒的核衣壳形成移动颗粒，在肌球蛋白 XI-K 驱动的肌动蛋白/内质网网络上运输。
DOI：
10.1111/nph.12447
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发表时间：
2013-12-01
期刊：
影响因子：
9.4
通讯作者：
Tao, Xiaorong
中科院分区：
文献类型：
--
作者：
Feng, Zhike;Chen, Xiaojiao;Tao, Xiaorong
植物病毒的许多病毒蛋白，除了运动蛋白，已被证明在细胞内沿着肌动蛋白丝运输并参与病毒感染。以番茄斑萎病毒（Tomato spotted wilt topovirus，TSWV）为模型病毒，研究了其核衣壳蛋白（Nucleocapsid，N）的细胞生物学特性，发现TSWV N蛋白能形成高度运动的胞质内含物，并能沿肌动蛋白和内质网（actin/actineticreticulum，ER）网络沿着移动。肌动蛋白解聚剂latrunculin B对肌动蛋白丝的破坏几乎阻止了N夹杂物的细胞内运动，而用微管解聚剂安磺灵处理则没有。肌球蛋白XI-K尾的过度表达，以显性负性的方式发挥作用，完全停止了N夹杂物的运动。Latrunculin B处理强烈抑制TSWV在烟草（Nicotiana tabacum cv Samsun NN）中的局部病变的形成，并延迟烟草（Nicotiana tabacum cv Samsun NN）的系统感染。总的来说，我们的发现提供了植物病毒衣壳蛋白具有细胞内运输的新特性的第一个证据。这些发现增加了衣壳蛋白作为一类新的病毒蛋白，它在肌动蛋白/ER系统上进行运输。
A number of viral proteins from plant viruses, other than movement proteins, have been shown to traffic intracellularly along actin filaments and to be involved in viral infection. However, there has been no report that a viral capsid protein may traffic within a cell by utilizing the actin/endoplasmic reticulum (ER) network.We used Tomato spotted wilt tospovirus (TSWV) as a model virus to study the cell biological properties of a nucleocapsid (N) protein.We found that TSWV N protein was capable of forming highly motile cytoplasmic inclusions that moved along the ER and actin network. The disruption of actin filaments by latrunculin B, an actin-depolymerizing agent, almost stopped the intracellular movement of N inclusions, whereas treatment with a microtubule-depolymerizing reagent, oryzalin, did not. The overexpression of a myosin XI-K tail, functioning in a dominant-negative manner, completely halted the movement of N inclusions. Latrunculin B treatment strongly inhibited the formation of TSWV local lesions in Nicotiana tabacum cv Samsun NN and delayed systemic infection in N. benthamiana.Collectively, our findings provide the first evidence that the capsid protein of a plant virus has the novel property of intracellular trafficking. The findings add capsid protein as a new class of viral protein that traffics on the actin/ER system.