Minimal tRNASer and tRNASec substrates for human seryl-tRNA synthetase:: contribution of tRNA domains to serylation and tertiary structure
Minimal tRNASer and tRNASec substrates for human seryl-tRNA synthetase:: contribution of tRNA domains to serylation and tertiary structure
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DOI：
10.1016/s0014-5793(98)00435-9
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发表时间：
1998-05-15
期刊：
影响因子：
3.5
通讯作者：
Gross, HJ
中科院分区：
文献类型：
--
作者：
Heckl, M;Busch, K;Gross, HJ
用代表人tRNA（Ser）或tRNA（Sec）特定区域的T7转录本研究了人丝氨酰-tRNA合成酶（SerRS）对tRNA（Ser）和tRNA（Sec）的识别过程，阐明了tRNA元件对丝氨酰化和三级结构的影响。D臂和T臂仅参与L形tRNA结构的形成，而不参与tRNA与SerRS的识别过程。这是首次报道人tRNA被其同源合成酶氨酰化的微螺旋，（C）1998年欧洲生物化学学会联合会。
The recognition process of tRNA(Ser) and tRNA(Sec) by human seryl-tRNA synthetase (SerRS) was studied using T7 transcripts representing defined regions of human tRNA(Ser) or tRNA(Sec) and the influence of the tRNA elements on serylation and tertiary structure was elucidated, The anticodon arms of both tRNAs showed no contribution to serylation in contrast to the acceptor stems and the long extra arms, D and T arms mere only involved in formation of the L-shaped tRNA structure, not in the recognition process between tRNAs and SerRS, This is the first report of microhelices adapted from human tRNAs being aminoacylated by their homologous synthetase, (C) 1998 Federation of European Biochemical Societies.