Individual differences in voluntary ethanol consumption lead to differential activation of the central amygdala in rats: relationship to the anxiolytic and stimulant effects of low dose ethanol.
Individual differences in voluntary ethanol consumption lead to differential activation of the central amygdala in rats: relationship to the anxiolytic and stimulant effects of low dose ethanol.
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DOI：
10.1111/j.1530-0277.2012.01907.x
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发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Wilson MA
中科院分区：
文献类型：
--
作者：
Sharko AC;Kaigler KF;Fadel JR;Wilson MA
虽然酒精使用障碍和焦虑症是高度共病的，但这两种疾病之间的关系尚未完全了解。我们实验室以前的工作表明，焦虑样行为在远交的Long-Evans大鼠中是高度可变的，并且与自愿乙醇消费水平有关，这表明基础焦虑状态影响乙醇摄入。为了进一步研究乙醇消耗的获得和焦虑表型之间的关系，Long-Evans大鼠在有限的饮酒范式中自愿乙醇消耗后评估了焦虑样行为和神经元激活。使用限制进入黑暗中饮用（DID）范例，允许大鼠自我施用乙醇（6%v/v）四天，并基于平均每日乙醇摄入量的中值分割将其分为高饮用组和低饮用组。在第四次饮用阶段后，立即在高架十字迷宫上测试动物并评估焦虑样行为。在中央和基底外侧杏仁核，以及终纹床核的Fos免疫反应性进行了评估。高酒精饮用者在十字迷宫的开放臂上花费的时间明显多于低酒精饮用者。高乙醇饮用者也增加了运动活动相比，低乙醇饮用者和饮水者。尽管Fos阳性细胞计数仅在中央杏仁核的高乙醇饮用者和低乙醇饮用者之间存在显着差异，但在所有检查的大脑区域中，Fos免疫反应性与乙醇消耗量呈正相关。我们的研究结果表明，远交大鼠将自愿消费行为有效剂量的乙醇在短期访问模型和乙醇消费与中央杏仁核的神经元激活增加呈正相关。
Although alcohol use disorders and anxiety disorders are highly comorbid, the relationship between these two disorders is not fully understood. Previous work from our laboratory shows that anxiety-like behavior is highly variable in outbred Long-Evans rats and is related to the level of voluntary ethanol consumption, suggesting that basal anxiety state influences ethanol intake. To further examine the relationship between the acquisition of ethanol consumption and anxiety phenotype, Long-Evans rats were assessed for anxiety-like behavior and neuronal activation following voluntary ethanol consumption in a limited access drinking paradigm. Rats were allowed to self-administer ethanol (6%v/v) for four days using a limited access drinking in the dark (DID) paradigm and divided into high and low drinking groups based on a median split of average daily ethanol intake. Immediately following the fourth drinking session, animals were tested on the elevated plus maze and evaluated for anxiety-like behaviors. Fos immunoreactivity was assessed in the central and basolateral amygdala, as well as the bed nucleus of the stria terminalis. High ethanol drinkers spent significantly more time on the open arms of the plus maze than low ethanol drinkers. High ethanol drinkers also had increased locomotor activity as compared to both low ethanol drinkers and water drinkers. Fos immunoreactivity was positively correlated with ethanol consumption in all brain regions examined, although Fos positive cell counts were only significantly different between high and low ethanol drinkers in the central amygdala. Our findings demonstrate that outbred rats will voluntarily consume behaviorally effective doses of ethanol in a short-term access model and ethanol consumption is positively correlated with increased neuronal activation in the central amygdala.