Noninvasive measurement of cerebral blood flow and glucose metabolic rate in the rat with high-resolution animal positron emission tomography (PET): a novel in vivo approach for assessing drug action in the brains of small animals.
Noninvasive measurement of cerebral blood flow and glucose metabolic rate in the rat with high-resolution animal positron emission tomography (PET): a novel in vivo approach for assessing drug action in the brains of small animals.
复制标题
利用高分辨率动物正电子发射断层扫描 (PET) 无创测量大鼠脑血流量和葡萄糖代谢率：一种评估小动物大脑药物作用的新型体内方法。
DOI：
10.1248/bpb.18.753
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发表时间：
1995
影响因子：
2
通讯作者：
H. Okada
中科院分区：
文献类型：
--
作者：
Y. Magata;H. Saji;S. Choi;K. Tajima;T. Takagaki;S. Sasayama;Y. Yonekura;H. Kitano;M. Watanabe;H. Okada
用我们最近开发的高分辨率动物正电子发射断层扫描（PET）系统无创地估计了大鼠脑葡萄糖代谢率（CMRglc）和脑血流量（CBF）。分别用放射性示踪剂18F-2-氟-2-脱氧葡萄糖和15 O-水尝试CMRglc和CBF的鉴定。根据PET获得的数据，在戊巴比妥麻醉下，预处理条件下的CMRglc和CBF值分别计算为51 +/- 14 mumol/min/100 g和61 +/- 7 ml/min/100 g。这些值与放射自显影技术获得的已发表数据非常一致。此外，静脉注射乙酰唑胺后，检测到CBF增加69%（平均值）。结果表明，这种高分辨率的动物PET扫描仪具有很大的潜力，在完整的和实验动物的药物相关的代谢和循环的影响进行评估。
The cerebral metabolic rate of glucose (CMRglc) and cerebral blood flow (CBF) in rats were estimated noninvasively with a high-resolution animal positron emission tomography (PET) system that we recently developed. Qualifications of CMRglc and CBF were attempted with radiotracers, 18F-2-fluoro-2-deoxyglucose and 15O-water, respectively. From the data obtained with PET, the CMRglc and CBF values under pretreatment conditions were respectively calculated as 51 +/- 14 mumol/min/100 g and 61 +/- 7 ml/min/100 g under pentobarbital-anesthesia. These values were in close agreement with published data obtained with the autoradiographic technique. Moreover, an increase of 69% (mean) in CBF was detected after i.v. acetazolamide administration. The results indicate that this high-resolution animal PET scanner holds great potential for the assessment of drug-related metabolic and circulatory effects in intact and experimental animals.