Genome-scale metabolic network of Cordyceps militaris useful for comparative analysis of entomopathogenic fungi.
Genome-scale metabolic network of Cordyceps militaris useful for comparative analysis of entomopathogenic fungi.
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DOI：
10.1016/j.gene.2017.05.027
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发表时间：
2017-08
期刊：
影响因子：
3.5
通讯作者：
Wanwipa Vongsangnak;Nachon Raethong;Warasinee Mujchariyakul;N. Nguyen;H. Leong;K. Laoteng
中科院分区：
文献类型：
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作者：
Wanwipa Vongsangnak;Nachon Raethong;Warasinee Mujchariyakul;N. Nguyen;H. Leong;K. Laoteng
构建了第一个代表蛹虫草全基因组规模代谢网络（iWV1170）的代谢网络，该网络由质膜、细胞质、线粒体、过氧化物酶体和细胞外空间五个区室的 894 个代谢物和 1,267 个代谢反应组成。 iWV1170 可用于解释其生长能力、虫草素和其他代谢物在各种底物上的产生的表型。 C 中存在大量编码用于降解复杂碳水化合物、脂质和蛋白质的胞外酶的基因。军事基因组。通过比较基因组规模分析，重建了假定的虫草素生物合成的腺嘌呤代谢途径，表明它们在十一种昆虫病原真菌中的进化关系。假定的虫草素生物合成所涉及的总体代谢途径也在 C 中得到了鉴定。蛹，包括中心碳代谢、氨基酸代谢（甘氨酸、L-谷氨酰胺和L-天冬氨酸）和核苷酸代谢（腺苷和腺嘌呤）。有趣的是，在 C 中观察到缺乏编码核糖核苷酸还原酶抑制剂的序列。军国主义可能会导致虫草素的过量生产。
The first genome-scale metabolic network ofCordyceps militaris(iWV1170) was constructed representing its whole metabolisms, which consisted of 894 metabolites and 1,267 metabolic reactions across five compartments, including the plasma membrane, cytoplasm, mitochondria, peroxisome and extracellular space. TheiWV1170 could be exploited to explain its phenotypes of growth ability, cordycepin and other metabolites production on various substrates. A high number of genes encoding extracellular enzymes for degradation of complex carbohydrates, lipids and proteins were existed inC. militarisgenome. By comparative genome-scale analysis, the adenine metabolic pathway towards putative cordycepin biosynthesis was reconstructed, indicating their evolutionary relationships across eleven species of entomopathogenic fungi. The overall metabolic routes involved in the putative cordycepin biosynthesis were also identified inC. militaris, including central carbon metabolism, amino acid metabolism (glycine,l-glutamine andl-aspartate) and nucleotide metabolism (adenosine and adenine). Interestingly, a lack of the sequence coding for ribonucleotide reductase inhibitor was observed inC. militaristhat might contribute to its over-production of cordycepin.