The Elongation Factor GmEF4 Is Involved in the Response to Drought and Salt Tolerance in Soybean
The Elongation Factor GmEF4 Is Involved in the Response to Drought and Salt Tolerance in Soybean
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伸长因子 GmEF4 参与大豆对干旱和耐盐性的反应
DOI：
10.3390/ijms20123001
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发表时间：
2019-06-02
影响因子：
5.6
通讯作者：
Ma, You-Zhi
中科院分区：
文献类型：
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作者：
Gao, Yuan;Ma, Jian;Ma, You-Zhi
越来越多的证据表明，伸长因子1 α (EF1 α)参与了几种植物对各种非生物胁迫的响应。大豆EF1 α蛋白包括三个结构域：一个gtp结合域和两个寡核苷酸结合域，也称为结构域2和结构域3。本研究在大豆基因组中鉴定了10个EF1 α基因。本研究预测了大豆EF1 α基因不同结构域的结构，分析了EF1 α基因的基因位置、基因结构、系统发育关系、各顺式元件和保守结构域，并利用实时荧光定量PCR （qRT-PCR）技术分析了10个EF1 α基因的表达模式。干旱胁迫下，大豆EF1 α基因均不同程度上调。特别是GmEF4在干旱和盐处理下表达上调。与干旱和盐处理的空载体（EV）对照植株相比，干旱和盐处理的gmef4过表达（OE）植株的叶片萎蔫时间显著延迟，根系更长，生物量更高，脯氨酸（Pro）含量更高，H2O2、O-2（-）和丙二醛（MDA）含量更低。因此，本研究为进一步研究这一重要家族在非生物胁迫下的功能基因组学奠定了基础。
Growing evidence indicates that elongation factor 1 alpha (EF1 alpha) is involved in responses to various abiotic stresses in several plant species. Soybean EF1 alpha proteins include three structural domains: one GTP-binding domain and two oligonucleotide binding domains that are also called as domain 2 and domain 3. In this study, 10 EF1 alpha genes were identified in the soybean genome. We predicted structures of different domains and analyzed gene locations, gene structures, phylogenetic relationships, various cis-elements, and conserved domains of soybean EF1 alpha s. The expression patterns of 10 EF1 alpha genes were analyzed by quantitative real-time PCR (qRT-PCR). Under drought stress, soybean EF1 alpha genes were upregulated in varying degrees. In particular, GmEF4 was upregulated under drought and salt treatments. Compared to the drought- and salt-treated empty vector (EV)-control plants, drought- and salt-treated GmEF4-overexpressing (OE) plants had significantly delayed leaf wilting, longer root, higher biomass, higher proline (Pro) content, and lower H2O2, O-2(-), and malondialdehyde (MDA) contents. Thus, this study provides a foundation for further functional genomics research about this important family under abiotic stress.