Stimuli-responsive disassembly of nanoparticle aggregates for light-up colorimetric sensing
Stimuli-responsive disassembly of nanoparticle aggregates for light-up colorimetric sensing
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DOI：
10.1021/ja053567u
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发表时间：
2005-09-14
影响因子：
15
通讯作者：
Lu, Y
中科院分区：
文献类型：
--
作者：
Liu, J;Lu, Y
纳米材料的可控组装一直是许多研究的焦点。相比之下，控制拆卸并没有得到太多的关注，即使这两个过程已被证明是重要的生物学。通过使用Pb2+依赖的RNA切割DNAzyme，我们在这里展示了响应于Pb2+的金纳米颗粒聚集体的分解的控制。在这个过程中，我们表明，纳米粒子的排列在拆卸过程中起着重要的作用，尾到尾的配置是最佳的，可能是因为其他配置的空间位阻大。通过使用小片段DNA侵入DNAzyme的切割底物，拆卸速率显著加快。这种受控拆卸过程的调查允许先前设计的"灯下"比色Pb2+传感器转化为"点亮"传感器。
Controlled assembly of nanomaterials has been the focus of much research. In contrast, controlled disassembly has not received much attention, even though both processes have been shown to be important in biology. By using a Pb2+-dependent RNA-cleaving DNAzyme, we demonstrate here control of the disassembly of gold nanoparticle aggregates in response to Pb2+. In the process, we show that nanoparticle alignment plays an important role in the disassembly process, with the tail-to-tail configuration being the most optimal, probably because of the large steric hindrance of other configurations. The rate of disassembly is significantly accelerated by using small pieces of DNA to invade the cleaved substrate of the DNAzyme. Investigation of such a controlled disassembly process allows the transformation of previously designed "light-down" colorimetric Pb2+ sensors into "light-up" sensors.