Assembled Plastid and Mitochondrial Genomes, as well as Nuclear Genes, Place the Parasite Family Cynomoriaceae in the Saxifragales.
Assembled Plastid and Mitochondrial Genomes, as well as Nuclear Genes, Place the Parasite Family Cynomoriaceae in the Saxifragales.
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DOI：
10.1093/gbe/evw147
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发表时间：
2016-08-03
影响因子：
3.3
通讯作者：
Renner SS
中科院分区：
文献类型：
--
作者：
Bellot S;Cusimano N;Luo S;Sun G;Zarre S;Gröger A;Temsch E;Renner SS
Cynomoriaceae是开花植物中最后一个未被放置的科之一，包括一个或两个种或亚种的根寄生虫，从地中海到戈壁沙漠。使用Illumina测序，我们组装了线粒体和质体基因组以及来自意大利的锁阳标本的一些核基因。通过桑格测序从中国和伊朗采集的个体中也获得了选定的基因，得到了33个线粒体、6个核和14个质体基因的矩阵，并根据GenBank中的数据将rDNAs扩大到包括代表性的被子植物分类群采样。我们还编制了一份新的地理地图，以识别寄生虫发生的可能不连续性。Cynomorium具有13.70-13.61（意大利）至13.95-13.76 pg（中国）的大基因组。它的线粒体基因组由多达49个环状亚基因组组成，其总体基因含量与光合被子植物相似，而其质体仅保留正常116个基因中的27个。核，质体和线粒体的同源性将Cynomoriaceae放在虎耳草目中，我们发现了来自不同宿主的几种水平基因转移以及细胞内基因转移的证据。
Cynomoriaceae, one of the last unplaced families of flowering plants, comprise one or two species or subspecies of root parasites that occur from the Mediterranean to the Gobi Desert. Using Illumina sequencing, we assembled the mitochondrial and plastid genomes as well as some nuclear genes of a Cynomorium specimen from Italy. Selected genes were also obtained by Sanger sequencing from individuals collected in China and Iran, resulting in matrices of 33 mitochondrial, 6 nuclear, and 14 plastid genes and rDNAs enlarged to include a representative angiosperm taxon sampling based on data available in GenBank. We also compiled a new geographic map to discern possible discontinuities in the parasites’ occurrence. Cynomorium has large genomes of 13.70–13.61 (Italy) to 13.95–13.76 pg (China). Its mitochondrial genome consists of up to 49 circular subgenomes and has an overall gene content similar to that of photosynthetic angiosperms, while its plastome retains only 27 of the normally 116 genes. Nuclear, plastid and mitochondrial phylogenies place Cynomoriaceae in Saxifragales, and we found evidence for several horizontal gene transfers from different hosts, as well as intracellular gene transfers.