GATA factors and the origins of adult and embryonic blood in Xenopus: Responses to retinoic acid
GATA factors and the origins of adult and embryonic blood in Xenopus: Responses to retinoic acid
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DOI：
10.1016/0925-4773(96)00547-3
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发表时间：
1996-07-01
影响因子：
2.6
通讯作者：
Patient, RK
中科院分区：
文献类型：
--
作者：
Bertwistle, D;Walmsley, ME;Patient, RK
转录因子 GATA-1、-2 和 -3 在造血细胞的分化中发挥重要作用。为了研究脊椎动物发育过程中的血液形成过程，我们利用这些 GATA 因子的表达来定位爪蟾胚胎中的造血细胞，并充当全反式视黄酸 (RA) 作用的传感器，全反式视黄酸 (RA) 是一种影响前后模式和造血分化的信号分子。在原肠胚形成中胚层的前缘、腹侧血岛 (VBI) 和背外侧板 (DLP) 中胚层以及 VBI 和 DLP 之间的细胞群中检测到 GATA 因子表达。 VBI 对胚胎和成人血液都有贡献，而 DLP 仅包含成人血液的前体，此前尚未通过分子标记进行鉴定。通过移植分析排除了VBI和DLP之间表达GATA-2的细胞是从VBI迁移到DLP的造血祖细胞的可能性。 RA对GATA-1和GATA-2表达的不同影响表明RA对造血分化有直接作用，而不是对造血中胚层的形成有作用。
The transcription factors, GATA-1, -2 and -3 play essential roles in the differentiation of haematopoietic cells. To study the process of blood formation during vertebrate development we have used the expression of these GATA factors to locate haematopoietic cells in Xenopus embryos and to act as sensors for the effects of all-trans retinoic acid (RA), a signalling molecule which influences both anteroposterior patterning and haematopoietic differentiation. GATA factor expression was detected in the leading edge of the gastrulating mesoderm, in the ventral blood island (VBI) and dorsolateral plate (DLP) mesoderms and in a population of cells between the VBI and DLP. The VBI contributes to both embryonic and adult blood, whereas the DLP contains precursors of adult blood only, which have not been identified previously with molecular markers. The possibility that the GATA-2-expressing cells between the VBI and DLP were haematopoietic progenitors migrating from the VBI to the DLP was ruled out by transplantation analysis. Differential effects of RA on the expression of GATA-1 and GATA-2 suggest that RA has a direct action on haematopoietic differentiation, rather than on the formation of haematopoietic mesoderm.