Interstitial Cajal-like cells of human Fallopian tube express estrogen and progesterone receptors
Interstitial Cajal-like cells of human Fallopian tube express estrogen and progesterone receptors
复制标题
DOI：
10.1007/s10735-009-9252-z
复制
发表时间：
2009-10-01
影响因子：
3.2
通讯作者：
Popescu, L. M.
中科院分区：
文献类型：
--
作者：
Cretoiu, Sanda Maria;Cretoiu, D.;Popescu, L. M.
雌性生殖道细胞通过在核水平表达的性类固醇基因组受体受到激素控制。我们以前发现，人子宫肌层的间质Cajal样细胞（ICLC）表达雌激素和孕激素受体（ER/PR）。基于这些结果，我们的目的是了解ER和/或PR是否也可在输卵管ICLC中发现。事实上，我们在这里提出的免疫组化证据表明，ICLC的人输卵管（峡部地区）有这样的受体。基质ICLC以及平滑肌层中的ICLC通过其形态学特征（例如，细胞体的几个非常长的念珠状突起）以及c-kit阳性、亚甲蓝或银浸渍活体染色在组织切片中鉴定。通过“夹心法”对同一细胞上的c-kit和PR进行连续染色，提供了额外的证据。在体外，通过免疫荧光法，显示ICLC形态的输卵管肌层的第4代细胞培养物显示ER-α和/或PR-A的存在。总之，我们的数据表明，ICLC可以作为类固醇传感器，并可能涉及输卵管运动（通过间隙连接或分泌和/或旁分泌机制）。
Cells of the female reproductive tract are subject to hormonal control via sex steroid genomic receptors expressed at nuclear level. We previously showed that interstitial Cajal-like cells (ICLC) of human myometrium expressed estrogen and progesterone receptors (ER/PR). Our aim, based on these results, was to see if ER and/or PR could be found also in tubal ICLC. Indeed, we present here immunohistochemical evidence that ICLC of human Fallopian tube (isthmic region) have such receptors. Stromal ICLC, as well as ICLC among smooth muscle layers, were identified in tissue sections by their morphological features (e.g. several very long, moniliform, prolongations of cell body) as well as by c-kit positivity, vital staining with methylene blue or silver impregnation. Additional evidence was provided by sequential staining for c-kit and for PR on the same cell, by 'sandwich method'. In vitro, the 4th passage cell cultures from Fallopian tube muscularis exhibiting ICLC morphology showed the presence of ER-alpha and/or PR-A by immunofluorescence. In conclusion, our data suggest that ICLC could function as steroid sensors, and might be implicated in Fallopian tube motility (via gap junctions or juxta- and/or paracrine mechanisms).