High efficiency gene transfer into primary human tumor explants without cell selection.
High efficiency gene transfer into primary human tumor explants without cell selection.
复制标题
无需细胞选择即可将基因高效转移至原代人类肿瘤外植体中。
作者：
E. Jaffee;G. Dranoff;L. Cohen;K. M. Hauda;S. Clift;F. Marshall;R. Mulligan;D. Pardoll
用鼠肿瘤模型进行的临床前研究已经证明，经工程改造以旁分泌方式分泌某些细胞因子的自体肿瘤细胞疫苗引起能够消除少量已建立肿瘤的全身免疫应答。这些结果引起了人们对发展这种人类癌症基因治疗策略的极大兴趣。产生遗传修饰的自体人肿瘤疫苗的主要限制是有效地将基因转移到原发性肿瘤外植体中，因为大多数人肿瘤在长期培养中不能增殖。使用逆转录病毒载体MFG结合短期培养技术，我们已经实现了，在没有选择的情况下，平均转导效率为60%，在原发性肾，卵巢和胰腺肿瘤外植体，我们已经开发了一种自体粒细胞-巨噬细胞集落刺激因子分泌肿瘤疫苗的临床试验。
Preclinical studies with murine tumor models have demonstrated that autologous tumor cell vaccines engineered to secrete certain cytokines in a paracrine fashion elicit systemic immune responses capable of eliminating small amounts of established tumor. These results have engendered much interest in developing this strategy for gene therapy of human cancer. The major limitation to creating genetically modified autologous human tumor vaccines is efficient gene transfer into primary tumor explants, since the majority of human tumors fail to proliferate in long-term culture. Using the retroviral vector MFG in conjunction with short-term culture techniques, we have achieved, in the absence of selection, a mean transduction efficiency of 60% in primary renal, ovarian, and pancreatic tumor explants, and we have developed an autologous granulocyte-macrophage colony-stimulating factor secreting tumor vaccine for clinical trials.
逆转录病毒载体介导的淋巴因子基因转移至人肾癌细胞中。
影响因子：
11.2
作者：
Gastl,G;Finstad,CL;Guarini,A;Bosl,G;Gilboa,E;Bander,NH;Gansbacher,B
通讯作者：
Gansbacher,B
逆转录病毒介导的基因转移作为分析自体骨髓移植后神经母细胞瘤复发的方法。
影响因子：
4.2
作者：
Rill,DR;Buschle,M;Foreman,NK;Bartholomew,C;Moen,RC;Santana,VM;Ihle,JN;Brenner,MK
通讯作者：
Brenner,MK