Mechanism of mutation on DNA templates containing synthetic abasic sites: study with a double strand vector.
Mechanism of mutation on DNA templates containing synthetic abasic sites: study with a double strand vector.
复制标题
含有合成脱碱基位点的 DNA 模板的突变机制：使用双链载体进行研究。
DOI：
10.1093/nar/22.10.1897
复制
发表时间：
1994
影响因子：
14.9
通讯作者：
Eisenberg,W
中科院分区：
文献类型：
--
作者：
Takeshita,M;Eisenberg,W
研究了基础位点的诱变。利用含有磷酸二酯主链上脱氧核糖合成类似物的双梭载体对大肠杆菌和猴肾（COS）细胞进行研究。病灶位于矢量的相反链上。当四氢呋喃基类似物作为基位时，在双链损伤部位引入AT或TA对（65-80%）。在没有SOS诱导的情况下发生诱变。单碱基缺失（bbb80 %）在缺乏环结构的丙基和乙基类似物的突变谱中占主导地位。在所有碱基类似物中，G/C和C/G对的比例都很小（6-10%）。dAMP主要与位于间隙双工载体单链区域的四氢呋喃基相反。我们从这些研究中得出结论，位于双链结构中的基本位点是高度诱变的基因。coliand COS细胞。修复DNA的合成可能参与了这一过程。
Mutagenesis at abasic sites was investigated inE.coliand simian kidney (COS) cells using a duplex shuttle vector containing synthetic analogs of deoxyribose on the phosphodiester backbone. Lesions were positioned on opposite strands of the vector. When the tetrahydrofuranyl analog was used as the abasic site, AT or TA pairs (65–80%) were introduced at the site of the bistrand lesion. Mutagenesis occurred in the absence of SOS induction. Single base deletions (>80%) dominated the mutational spectra for propanyl and ethanyl analogs of abasic sites lacking a ring structure. For all abasic site analogs, a small proportion of G/C and C/G pairs (6–10%) were observed. dAMP was incorporated predominantly opposite tetrahydrofuranyl sites positioned in the single strand region of a gapped duplex vector. We conclude from these studies that abasic sites positioned in a bistrand configuration are highly mutagenic inE.coliand COS cells. Repair DNA synthesis may be involved in this process.