In vitro activation of the in vivo colony‐forming units of the mouse yolk sac
In vitro activation of the in vivo colony‐forming units of the mouse yolk sac
复制标题
小鼠卵黄囊体内集落形成单位的体外激活
DOI：
10.1002/jcp.1040910205
复制
发表时间：
1977
影响因子：
5.6
通讯作者：
M. Feldman
中科院分区：
文献类型：
--
作者：
G. Perah;M. Feldman
Experiments were performed to investigate the presence of colony‐forming units (CFU) in the mouse embryonic yolk sac during the developmental period in which the yolk sac is the sole hemopoietic organ. Injection of yolk sac cell suspensions from normal embryos into syngeneic, lethally irradiated adult recipients evoked a very low number of spleen colonies. However, prior cultivation of yolk sacs in vitro caused a dramatic increase in the spleen colony‐forming capacity–as high as 84‐fold–following 48 hours in culture. The yolk sac origin of the spleen colonies was confirmed by: (a) Chromosomal marker analysis; (b) dose‐response analysis; (c) demonstrating that the above colonies were not of endogenous origin induced by the mere injection of grafted cells. We conclude that the yolk sac contains many precursors of colony‐forming cells which though undetectable by immediate grafting apparently become activated in culture by an as yet unknown induction process.
