Non-destructive electromechanical assessment (Arthro-BST) of human articular cartilage correlates with histological scores and biomechanical properties
Non-destructive electromechanical assessment (Arthro-BST) of human articular cartilage correlates with histological scores and biomechanical properties
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DOI：
10.1016/j.joca.2014.08.008
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发表时间：
2014-11-01
影响因子：
7
通讯作者：
Buschmann, M. D.
中科院分区：
文献类型：
--
作者：
Sim, S.;Chevrier, A.;Buschmann, M. D.
目的：应用手持式关节- bst （TM）仪测量关节软骨的机电特性。本研究的目的是评估软骨的组织学、生化和生物力学性能与机电性能的相关性。方法：采用关节- bst（1测量/位点，5秒/位点，3209个位点）对8例人体远端股骨的机电特性（定量参数（QP））进行人工离体测绘。然后从股骨的不同区域采集骨软骨核并用Mankin组织学评分进行评估。在组织处理之前，岩心在无侧限压缩下进行测试。用偏振光显微镜（PLM）分析核的一个子集来评估胶原结构。对另外的岩心进行生化测定以获得水分和糖胺聚糖（GAG）的含量。每个岩心对应的QP是通过平均岩心中心6mm范围内收集的所有QP来计算的。结果：机电QP与Mankin评分和PLM评分均有较强相关性（r = 0.73, P < 0.0001和r = -0.70, P < 0.0001），能准确反映组织质量和胶原结构。机电QP还与生物力学性能密切相关，包括纤维模量（r = -0.76, P < 0.0001）、基质模量（r = -0.69, P < 0.0001）和渗透率对数（r = 0.72, P < 0.0001）。QP与每湿重GAG和水分含量呈弱相关（r = -0.50, P < 0.0003, r = 0.39, P < 0.006）。结论：非破坏性机电QP测量与组织学评分和生物力学参数密切相关，可快速可靠地评估关节软骨质量。(C) 2014年国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objective: The hand-held Arthro-BST (TM) device is used to map electromechanical properties of articular cartilage. The purpose of the study was to evaluate correlation of electromechanical properties with histological, biochemical and biomechanical properties of cartilage.Method: Electromechanical properties (quantitative parameter (QP)) of eight human distal femurs were mapped manually ex vivo using the Arthro-BST (1 measure/site, 5 s/measure, 3209 sites). Osteochondral cores were then harvested from different areas on the femurs and assessed with the Mankin histological score. Prior to histoprocessing, cores were tested in unconfined compression. A subset of the cores was analyzed with polarized light microscopy (PLM) to assess collagen structure. Biochemical assays were done on additional cores to obtain water content and glycosaminoglycan (GAG) content. The QP corresponding to each core was calculated by averaging all QPs collected within 6 mm of the core center.Results: The electromechanical QP correlated strongly with both the Mankin score and the PLM score (r = 0.73, P < 0.0001 and r = -0.70, P < 0.0001 respectively) thus accurately reflecting tissue quality and collagen architecture. Electromechanical QP also correlated strongly with biomechanical properties including fibril modulus (r = -0.76, P < 0.0001), matrix modulus (r = -0.69, P < 0.0001), and log of permeability (r = 0.72, P < 0.0001). The QP correlated weakly with GAG per wet weight and with water content (r = -0.50, P < 0.0003 and r = 0.39, P < 0.006 respectively).Conclusion: Non-destructive electromechanical QP measurements correlate strongly with histological scores and biomechanical parameters providing a rapid and reliable assessment of articular cartilage quality. (C) 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.