Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis
Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis
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DOI：
10.1007/s00428-005-0136-7
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发表时间：
2006-04-01
期刊：
影响因子：
3.5
通讯作者：
Arakawa, T
中科院分区：
文献类型：
--
作者：
Jinno, Y;Ohtani, H;Arakawa, T
溃疡性结肠炎的发病与体液免疫功能低下有关。然而，B细胞活化的详细机制在该地区仍然不明。我们从局部淋巴细胞扩张的角度分析溃疡性结肠炎。通过免疫组织化学和流式细胞术分析了30例接受皮质类固醇治疗的溃疡性结肠炎患者和15例克罗恩病患者手术时获得的肠道标本。溃疡性结肠炎的特征是Ki-67（+）小圆细胞弥漫性分布，特别是在溃疡基底（即CD 19（+）和CD 20（-）），其中大量细胞也为CD 138（+）。CD 19的免疫电镜显示了丰富的粗面内质网在细胞质中。这些表明它们是未成熟的浆系细胞。相比之下，克罗恩病的浆细胞CD 19阴性，Ki-67标记很少，显示成熟的表型。流式细胞术显示溃疡性结肠炎中存在CD 19（+）和CD 20（-）细胞，但克罗恩病中不存在。CD 19（+）浆细胞Ki-67标记指数与溃疡性结肠炎临床活动性呈正相关。CD 19（+）浆细胞中Ki-67的高标记是对皮质类固醇药物治疗耐药的活动性溃疡性结肠炎的特异性标志。
Abnormalities in humoral immunity are implicated in the pathogenesis of ulcerative colitis. However, the detailed mechanisms of B-cell activation in the locale remain unaccounted for. We analyzed ulcerative colitis from the standpoint of lymphocytic expansion in the loco. Intestinal specimens obtained at surgery from 30 patients with ulcerative colitis treated with corticosteroids and 15 with Crohn's disease were analyzed by immunohistochemistry and flow cytometry. Ulcerative colitis was characterized by a diffuse distribution of Ki-67(+) small round cells particularly in the ulcer base ( that were CD19(+) and CD20(-)), with a significant number of them also CD138(+). Immunoelectron microscopy for CD19 revealed an abundance of rough endoplasmic reticulum in the cytoplasm. These indicated that they are of immature plasma lineage cells. By contrast, plasma cells in Crohn's disease were negative for CD19, and the labeling for Ki-67 was infrequent, showing mature phenotype. Flow cytometry revealed an occurrence of CD19(+) and CD20(-) cells in ulcerative colitis but not in Crohn's disease. The labeling index of Ki-67 among CD19(+) plasma cells was positively correlated with the clinical activity of ulcerative colitis. High labeling of Ki-67 in CD19(+) plasma cells is specific for active ulcerative colitis that was resistant to medical treatment by corticosteroids.