Evaluation of the INNOVANCE PFA P2Y test cartridge: Sensitivity to P2Y12 blockade and influence of anticoagulant
Evaluation of the INNOVANCE PFA P2Y test cartridge: Sensitivity to P2Y12 blockade and influence of anticoagulant
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DOI：
10.3109/09537104.2011.601361
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发表时间：
2012-01-01
期刊：
影响因子：
3.3
通讯作者：
Harrison, Paul
中科院分区：
文献类型：
--
作者：
Edwards, Abbie;Jakubowski, Joseph A.;Harrison, Paul
血小板ADP受体P2 Y（12）抑制的监测可通过多种血小板功能测定进行。鉴于现有PFA-100（R）药筒配方检测P2 Y（12）抑制缺乏灵敏度，最近开发了一种用于PFA-100的新药筒（INNOVANCE（R）PFA P2 Y）。将新型PFA-100检测盒的性能与标准胶原/ADP（CADP）和胶原/肾上腺素（CEPI）检测盒、光透射聚集测定法、血管舒张剂刺激磷蛋白、VerifyNow（R）P2 Y（12）测定法和多电极聚集测定法进行了比较。在这项研究中，20份用柠檬酸盐或水蛭素抗凝的正常血液样本在37 ℃下加入两种不同临床相关浓度（1 μ M和10 μ M终浓度）的普拉格雷活性代谢物（R-138727，Lilly/Daiichi Sankyo）30分钟。血小板功能试验的比较表明，10 μ M R-138727基本上抑制了所有试验（CADP和CEPI除外）。通常在柠檬酸盐血液中使用1 μ M R-138727获得中间结果。然而，MEA ADP和ADPHS试验对水蛭素抗凝血中的1 μ M R-138727高度敏感。柠檬酸盐或水蛭素血样（N 5）的进一步比较显示，所有血小板试验（CEPI除外）对水蛭素化血液中的1 μ M R-138727变得更加敏感。经证明，INNOVANCE PFA P2 Y测试卡片对P2 Y（12）抑制敏感，且与目前可用的其他血小板功能检测试剂盒相当。检测P2 Y体外抑制的所有血小板功能试验的灵敏度（12）根据所用抗凝剂的不同而明显不同。
Monitoring of platelet ADP receptor P2Y(12) inhibition may be performed by a variety of platelet function assays. Given the lack of sensitivity of the existing PFA-100 (R) cartridge formulations to detect P2Y(12) inhibition, a new cartridge for the PFA-100 (INNOVANCE (R) PFA P2Y) has recently been developed. The performance of the new PFA-100 test cartridge was compared with standard collagen/ADP (CADP) and collagen/epinephrine (CEPI) cartridges, light transmission aggregometry, vasodilator-stimulated phosphoprotein, the VerifyNow (R) P2Y(12) assay and multiple electrode aggregometry. In this study, 20 normal blood samples anticoagulated with either citrate or hirudin were spiked with two different clinically relevant concentrations (1 and 10 mu M final concentration) of the prasugrel active metabolite (R-138727, Lilly/Daiichi Sankyo) for 30 min at 37 degrees C. Comparison of the platelet function tests demonstrated that all tests (except CADP and CEPI) were substantially inhibited by 10 mu M R-138727. Intermediate results were typically obtained with 1 mu M R-138727 in citrated blood. However, both MEA ADP and ADPHS tests were highly sensitive to 1 mu M R-138727 in hirudin anticoagulated blood. Further comparison of citrate or hirudin blood samples (N 5) revealed that all platelet tests (except CEPI) became more sensitive to 1 mu M R-138727 in hirudinized blood. The INNOVANCE PFA P2Y cartridge proved to be sensitive to P2Y(12) inhibition and was comparable to other currently available platelet function tests. The sensitivity of all platelet function tests for detecting in vitro inhibition of P2Y(12) is markedly different depending on the anticoagulant used.