Spectroelectrochemical behavior of parallel arrays of single vertically oriented Pseudomonas aeruginosa cells.
Spectroelectrochemical behavior of parallel arrays of single vertically oriented Pseudomonas aeruginosa cells.
复制标题
单个垂直方向的铜绿假单胞菌细胞平行阵列的光谱电化学行为。
DOI：
10.1016/j.xcrp.2023.101368
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Bohn,PaulW
中科院分区：
文献类型：
--
作者：
Cutri,AllisonR;Sundaresan,Vignesh;Shrout,JoshuaD;Bohn,PaulW
铜绿假单胞菌是一种革兰氏阴性机会性人类病原体，可引起多种医疗保健相关感染。评估 P 的单细胞贡献。 铜绿假单胞菌抗生素耐药性或毒力是当前的一个挑战，因为机械行为通常是从群体培养物中推断出来的，从而平均了对人群的影响。在这里，我们开发并表征了一种可以捕获 singleP 阵列的设备。 单个微孔中的铜绿假单胞菌，以研究其光谱电化学行为。制造了一系列单独的微孔，其尺寸旨在捕获 singleP。 铜绿假单胞菌呈垂直方向。将电化学势波形应用到底部金层后，可以从对外部条件敏感的捕获细胞中观察到三种特征荧光行为。这里开发的设备能够研究单个细菌细胞的平行收集，并将有助于阐明单细胞水平上的细菌通讯和多药耐药性的方法。
Pseudomonas aeruginosais a Gram-negative opportunistic human pathogen responsible for a number of healthcare-associated infections. Assessing single-cell contributions ofP. aeruginosato antibiotic resistance or virulence is a current challenge, because mechanistic behavior is conventionally inferred from ensemble cultures, thus averaging effects over a population. Here, we develop and characterize a device that can capture arrays of singleP. aeruginosacells in individual micropores to study their spectroelectrochemical behavior. An array of individual micropores is fabricated with dimensions designed to capture singleP. aeruginosacells in a vertical orientation. Upon application of an electrochemical potential waveform to the bottom Au layer, three characteristic fluorescence behaviors are observed from trapped cells that are sensitive to external conditions. The device developed here enables the study of parallel collections of single bacterial cells and will facilitate efforts to elucidate methods of bacterial communication and multidrug resistance at the single-cell level.