Thermophilic helicase-dependent DNA amplification using the IsoAmp™ SE experimental kit for rapid detection of Streptococcus equi subspecies equi in clinical samples
Thermophilic helicase-dependent DNA amplification using the IsoAmp™ SE experimental kit for rapid detection of Streptococcus equi subspecies equi in clinical samples
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DOI：
10.1177/1040638711416968
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发表时间：
2011-09-01
影响因子：
1.5
通讯作者：
Kong, Huimin
中科院分区：
文献类型：
--
作者：
Artiushin, Sergey;Tong, Yanhong;Kong, Huimin
建立了一种简便、快速的检测马链球菌马亚种的方法。使用嗜热解旋酶依赖性反应进行等特异性测序，然后在一次性侧向流动盒中目视检测扩增子。评价了实验试剂盒（IsopropylTM SE）。分析灵敏度为50个拷贝的S。马基因组DNA/反应。当应用于鼻拭子和洗涤液时，IsopropylSE测定具有100%的特异性。该方法比培养法敏感，但不如巢式聚合酶链反应（PCR）敏感。该检测既不需要昂贵的设备，也不需要对人员进行广泛的培训，为检测S.马沙门氏菌的非典型菌落的鉴定。马链球菌和动物链球菌。
A simple and portable assay for detection of Streptococcus equi subspecies equi has been developed based on amplification of S. equi-specific sequence using a thermophilic helicase-dependent reaction followed by visual detection of the amplicon in a disposable lateral flow cassette. An experimental kit (IsoAmp (TM) SE) was evaluated. Analytical sensitivity was 50 copies of S. equi genomic DNA per reaction. The IsoAmp SE assay had 100% specificity when applied to nasal swabs and washes. The assay was more sensitive than culture but less sensitive than nested polymerase chain reaction (PCR). The test requires neither expensive equipment nor extensive training of personnel, provides a practical alternative to culture or PCR assays for detection of S. equi in clinical samples, and expedites identification of atypical colonies of S. equi and Streptococcus zooepidemicus in the laboratory.