Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9.
Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9.
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具有 O4 抗原和 O9 抗原的沙门氏菌菌株脂多糖的结构和免疫化学研究。
DOI：
10.1128/jb.174.6.1916-1922.1992
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发表时间：
1992
影响因子：
3.2
通讯作者：
Lindberg,AA
中科院分区：
文献类型：
--
作者：
Weintraub,A;Johnson,BN;Stocker,BA;Lindberg,AA
两种沙门氏菌杂交菌株，SL 5313（具有D.rfb+基因簇的鼠伤寒沙门氏菌）和SL 5396（S.使用O 4特异性小鼠单克隆抗体和O 9特异性兔多克隆抗体的混合物，通过免疫荧光研究了各自表达O-抗原4（血清群B）和O-抗原9（血清群D）的具有B.rfb+基因簇的大肠杆菌。结合抗体，检测标记的抗鼠抗体与异硫氰酸荧光素和标记的抗兔抗体与四甲基罗丹明异硫氰酸盐表明，超过98%的细菌表达的O 4和O 9表位。通过气液色谱和质谱法对来自分批生长培养物的苯酚-水提取的脂多糖进行糖和甲基化分析，结果显示，对于SL 5313和SL 5396，分别以1：1.5和1：2.5的比例含有阿贝可糖（O 4表位）和泰维糖（O 9表位）。分离的多糖链，通过弱酸水解的脂多糖，被发现包含O 4和O 9特异性在同一分子中，因为多糖结合到O 4抗体附着到固相吸附的O 9特异性抗体，反之亦然。这表明在菌株SL 5313和SL 5396中，含有两个O 4重复单元的O链（来自S.鼠伤寒沙门氏菌）和O 9单位（来自S.）都在场。
Two Salmonella hybrid strains, SL5313 (Salmonella typhimurium with a D.rfb+ gene cluster) and SL5396 (S. enteritidis with a B.rfb+ gene cluster), each expressing both O-antigen 4 (of serogroup B) and O-antigen 9 (of serogroup D) were studied by immunofluorescence using a mixture of O4-specific mouse monoclonal and O9-specific rabbit polyclonal antibodies. Bound antibodies, detected by anti-mouse antibody labelled with fluorescein isothiocyanate and anti-rabbit antibody labelled with tetramethylrhodamine isothiocyanate showed that more than 98% of the bacteria expressed both the O4 and O9 epitopes. Phenol-water-extracted lipopolysaccharide from batch-grown cultures subjected to sugar and methylation analyses by gas-liquid chromatography and mass spectrometry were shown to contain abequose (of the O4 epitope) and tyvelose (of the O9 epitope) in ratios of 1:1.5 and 1:2.5 for SL5313 and SL5396, respectively. Isolated polysaccharide chains, obtained by weak-acid hydrolysis of the lipopolysaccharides, were found to contain both O4 and O9 specificities in the same molecule, since polysaccharide bound to O4 antibody attached to a solid-phase-adsorbed O9-specific antibody and vice versa. This demonstrates that in strains SL5313 and SL5396 O chains containing both O4 repeating units (from S. typhimurium) and O9 units (from S. enteritidis) are present.