AIE-Active Fluorescent Nonconjugated Polymer Dots for Dual-Alternating-Color Live Cell Imaging
AIE-Active Fluorescent Nonconjugated Polymer Dots for Dual-Alternating-Color Live Cell Imaging
复制标题
用于双色交替活细胞成像的 AIE 活性荧光非共轭聚合物点
DOI：
10.1021/acs.iecr.8b03776
复制
发表时间：
2018-11-07
影响因子：
4.2
通讯作者：
Lei, Ziqiang
中科院分区：
文献类型：
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作者：
Guan, Xiaolin;Lu, Baocui;Lei, Ziqiang
微调发射荧光生物探针是非常可取的，但对于双交替彩色活细胞成像来说仍然很少。在这里，已经报道了一种简便的策略，即开发一种用于靶向癌细胞成像的双色纳米探针。通过两亲四臂星形聚合物TPE-TPNIPAM-Eu(III)在水介质中的溶液自组装，制备了一种新型的非共轭聚合物点(Pdots)。所制备的Pdots具有两个波长相差195 nm的发射峰，并具有荧光-热响应特性，其LC_(ST)为37°C。此外，Pdots的高效细胞摄取和低细胞毒性有利于其在活细胞成像中的应用。细胞成像研究表明，只需调节激发波长，就可以很容易地在三个带有Pdots荧光标记的癌细胞中实现可光开关的双发射。因此，这一研究可能有助于开辟一条新的可行的道路。
Fine-tuning emission fluorescent bioprobes are highly desirable but still scarce for dual-alternating-color live cell imaging. Herein, a facile strategy has been reported to develop a dual-color nanoprobe for targeted cancer cell imaging. A new kind of nonconjugated polymer dots (Pdots) is prepared by the solution self-assembly of an amphiphilic 4-arm star polymer TPE-tetraPNIPAM-Eu(III) in aqueous media. The as-prepared Pdots show two well-separated emission peaks with a wavelength difference of 195 nm and possess a fluorescent-thermoresponsive property with a LCST at 37 °C. Furthermore, the efficient cellular uptake and low cytotoxicity of Pdots can be favorable for their applications in live cell imaging. The investigation of cellular imaging indicates that the photoswitchable dual-emission could be easily realized in targeted three cancer cells with a fluorescent label of Pdots by merely tuning the excitation wavelength. Therefore, this research may be of great help to open up a new and feasible path ...