INTRINSIC AND EXTRINSIC FACTORS IN PROTEIN ANTIGENIC STRUCTURE
INTRINSIC AND EXTRINSIC FACTORS IN PROTEIN ANTIGENIC STRUCTURE
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DOI：
10.1126/science.2410982
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
BERZOFSKY, JA
中科院分区：
文献类型：
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作者：
BERZOFSKY, JA
近年来，在合成多肽疫苗的制备和合成多肽作为抗原结构和功能的探针方面的进展，重新引起了人们对抗体和T细胞识别的抗原位点预测的兴趣。本文主要介绍抗体的研究进展。抗原固有的特性，如亲水性和流动性，在选择天然蛋白质的氨基酸序列时可能是有用的，这些氨基酸序列将引起抗体与多肽的交叉反应，或者作为多肽的序列，将更有可能引起抗体与天然蛋白的交叉反应。一般而言，结构流动性也可能有助于蛋白质之间的相互作用。然而，蛋白质的整个可接近表面很可能被足够大的抗体小组检测到。这些抗体中的哪一种是在任何个体身上制造的，取决于抗原分子的外部因素、宿主因素，如自身耐受性、免疫反应基因、独特型网络和免疫球蛋白结构基因库。
Recent advances in the preparation of synthetic peptide vaccines and the use of synthetic peptides as probes of antigenic structure and function have led to renewed interest in the prediction of antigenic sites recognized by antibodies and T cells. This review focuses on antibodies. Features intrinsic to the antigen, such as hydrophilicity and mobility, may be useful in the selection of amino acid sequences of the native protein that will elicit antibodies cross-reacting with peptides, or sequences which, as peptides, will be more likely to elicit antibodies cross-reactive with the native protein. Structural mobility may also contribute to protein-protein interactions in general. However, the entire accessible surface of a protein is likely to be detectable by a large enough panel of antibodies. Which of these antibodies are made in any individual depends on factors extrinsic to the antigen molecule, host factors such as self-tolerance, immune response genes, idiotype networks, and the immunoglobulin structural gene repertoire.