Titanium Dioxide Nanoparticles Relieve Silk Gland Damage and Increase Cocooning of Bombyx mori under Phoxim-Induced Toxicity
Titanium Dioxide Nanoparticles Relieve Silk Gland Damage and Increase Cocooning of Bombyx mori under Phoxim-Induced Toxicity
复制标题
二氧化钛纳米颗粒可减轻辛硫磷引起的毒性下的丝腺损伤并增加家蚕的结茧能力
DOI：
10.1021/jf4039259
复制
发表时间：
2013-12-18
影响因子：
6.1
通讯作者：
Hong, Fashui
中科院分区：
文献类型：
--
作者：
Li, Bing;Yu, Xiaohong;Hong, Fashui
有机磷农药在世界范围内被广泛应用于农业用途，在中国地区，有机磷农药的暴露常常导致家蚕不结茧。已有研究表明，纳米二氧化钛可以提高家蚕的抗药性。虽然辛硫磷的毒性已经有了很好的记录，但关于纳米二氧化钛改善辛硫磷暴露后家蚕结茧功能的机制的信息非常有限。因此，本研究旨在确定纳米二氧化钛是否能减轻辛硫磷暴露后家蚕的丝腺损伤并提高其结茧能力。结果表明，辛硫磷暴露对家蚕丝腺结构造成严重破坏，显着降低家蚕丝腺的结茧能力。此外，辛硫磷暴露显著降低了丝腺中总蛋白浓度，抑制了丝蛋白合成相关基因的表达，包括Fib-L、Fib-H P25、Ser-2和Ser-3。然而，纳米二氧化钛可以显著减轻家蚕丝腺的损伤。重要的是，纳米二氧化钛能显著提高蚕丝结茧和总蛋白含量，并促进辛硫磷暴露后丝腺中Fib-L、Fib-H、P25、Ser2和Ser3的表达。
Organophosphate pesticides are applied widely in the world for agricultural purposes, and their exposures often resulted in non-cocooning of Bombyx mori in China. TiO2 nanoparticles have been demonstrated to increase pesticide resistance of Bombyx mori. While the toxicity of phoxim is well-documented, very limited information exists on the mechanisms of TiO2 nanoparticles improving the cocooning function of Bombyx mori following exposure to phoxim. The present study was, therefore, undertaken to determine whether TiO2 nanoparticles attenuate silk gland injury and elevate cocooning of B. mori following exposure to phoxim. The findings suggested that phoxim exposure resulted in severe damages of the silk gland structure and significantly decreased the cocooning in the silk gland of Bombyx mori. Furthermore, phoxim exposure significantly resulted in reductions of total protein concentrations and suppressed expressions of silk protein synthesis-related genes, including Fib-L, Fib-H P25, Ser-2, and Ser-3, in the silk gland. TiO2 nanoparticle pretreatment, however, could significantly relieve silk gland injury of Bombyx mori. Importantly, TiO2 nanoparticles could remarkably elevate cocooning and total protein contents and promote expressions of Fib-L, Fib-H, P25, Ser-2, and Ser-3 in the silk gland following exposure to phoxim.