Identification and functional analysis of CG3526 in spermatogenesis of Drosophila melanogaster
Identification and functional analysis of CG3526 in spermatogenesis of Drosophila melanogaster
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DOI：
10.1111/1744-7917.13243
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发表时间：
2023-07-18
期刊：
影响因子：
4
通讯作者：
Yu，Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Hu，Qihao;Xiao，Yanhong;Yu，Xiao-Qiang
Spermatogenesis is a critical part of reproduction in insects; however, its molecular mechanism is still largely unknown. In this study, we identified a testis‐specific geneCG3526inDrosophila melanogaster. Bioinformatics analysis showed that CG3526 contains a zinc binding domain and 2 C2H2type zinc fingers, and it is clustered to the vertebrate really interesting new gene (RING) family E3 ubiquitin‐protein ligases. WhenCG3526was knocked down by RNA interference (RNAi), the testis became much smaller in size, and the apical tip exhibited a sharp and thin end instead of the blunt and round shape in the control testis. More importantly, compared to the control flies, only a few mature sperm were present in the seminal vesicle ofC587‐Gal4 > CG3526RNAi flies. Immunofluorescence staining of the testis fromCG3526RNAi flies showed that the homeostasis of testis stem cell niche was disrupted, cell distribution in the apical tip was scattered, and the process of spermatogenesis was not completed. Furthermore, we found that the phenotype ofCG3526RNAi flies’ testis was similar to that of testis of Stat92E RNAi flies, the expression level ofCG3526was significantly downregulated in theStat92EF06346mutant flies, and the promoter activity ofCG3526was upregulated by STAT92E. Taken together, our results indicated thatCG3526is a downstream effector gene in the JAK‐STAT signaling pathway that plays a key role in the spermatogenesis ofDrosophila.
