The role of GW/P-bodies in RNA processing and silencing
The role of GW/P-bodies in RNA processing and silencing
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DOI：
10.1242/jcs.03429
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发表时间：
2007-04-15
影响因子：
4
通讯作者：
Chan, Edward K. L.
中科院分区：
文献类型：
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作者：
Jakymiw, Andrew;Pauley, Kaleb M.;Chan, Edward K. L.
GW体，也称为哺乳动物P-体，是参与真核基因表达的转录后调节的细胞质灶。最近，GW体被证实与RNA干扰有关，并在短干扰RNA和microRNA介导的mRNA降解和翻译抑制中起重要作用.证据表明，短干扰RNA双链体的过客链和引导链都可以定位于GW体，从而表明RNA诱导的沉默复合物可以在这些细胞质中心内被激活。GW体的形成似乎取决于特定的蛋白质因子和RNA，特别是microRNA。过去几年的工作大大增加了我们对GW体生物学的理解，揭示了它们是在各种生物过程中空间调节mRNA周转的专门细胞组分。GW体的形成似乎取决于特定的蛋白质因子和RNA，特别是microRNA。在这里，我们提出了一个工作模型GW体组装在其关系RNA干扰。在这个过程中，一个或多个异聚体蛋白质复合物在连续的步骤中积累成更大的核糖核蛋白结构。
GW bodies, also known as mammalian P- bodies, are cytoplasmic foci involved in the post- transcriptional regulation of eukaryotic gene expression. Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short- interfering- RNAand microRNA- mediated mRNA decay and translational repression. Evidence indicates that both passenger and guide strands of short- interfering RNA duplexes can localize to GW bodies, thereby indicating that RNA-induced silencing complexes may be activated within these cytoplasmic centers. Formation of GW bodies appears to depend on both specific protein factors and RNA, in particular, microRNA. Work over the past few years has significantly increased our understanding of the biology of GW bodies, revealing that they are specialized cell components that spatially regulate mRNA turnover in various biological processes. The formation of GW bodies appears to depend on both specific protein factors and RNA, in particular, microRNA. Here, we propose a working model for GW body assembly in terms of its relationship to RNA interference. In this process, one or more heteromeric protein complexes accumulate in successive steps into larger ribonucleoprotein structures.