ALVEOLAR MACROPHAGE ACCESSORY CELL-FUNCTION IN BRONCHIAL-ASTHMA
ALVEOLAR MACROPHAGE ACCESSORY CELL-FUNCTION IN BRONCHIAL-ASTHMA
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DOI：
10.1164/ajrccm/146.4.900
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发表时间：
1992-10-01
期刊：
影响因子：
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通讯作者：
HAMBLIN, AS
中科院分区：
文献类型：
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作者：
GANT, V;CLUZEL, M;HAMBLIN, AS
本文研究了11例哮喘患者和11例正常人支气管肺泡灌洗液（BAL）提取的外周血单核细胞和肺泡巨噬细胞（AM）对结核菌素纯蛋白衍生物（PPD）或链激酶-链道酶（SKSD）的回忆性抗原的提呈能力。 在哮喘组中，AM辅助细胞功能是可变的，大多数受试者不能有效地呈现回忆抗原，血液单核细胞，虽然一个哮喘受试者表现出更大的增殖反应比血液单核细胞的两种抗原。 AM辅助细胞活性不是抗原特异性的，并且辅助细胞对两种抗原的功效之间存在相关性（r = 0.92;置信区间，0.53至0.98）。 此外，对于PPD（r = 0.92; 95%置信区间，0.83至0.99）和SKSD（r = 0.90; 95%置信区间，0.45至0.98），BAL液中淋巴细胞计数百分比与巨噬细胞与单核细胞抗原呈递能力比值之间存在相关性。 在正常人中，大多数受试者AM也不能有效地作为PPD和SKSD的辅助细胞。 支气管肺泡灌洗液中淋巴细胞百分比与AM/单核细胞辅助细胞功能比值无相关性。 这些结果提示支气管哮喘中AM辅助功能与BAL淋巴细胞的存在相关。
The capacity of peripheral blood monocytes and alveolar macrophages (AM) obtained by bronchoalveolar (BAL) to present recall antigens, namely, tuberculin purified protein derivative (PPD) or streptokinase-streptodornase (SKSD), to highly purified autologous T-cells has been studied in 11 asthmatic and 11 healthy, nonatopic normal subjects. In the asthmatic group, AM accessory cell function was variable, and most subjects were unable to present either recall antigen as effectively as blood monocytes, although one asthmatic subject demonstrated larger proliferative responses than blood monocytes for both antigens. AM accessory cell activity was not antigen-specific and there was a correlation between accessory cell efficacy for the two antigens (r = 0.92; confidence interval, 0.53 to 0.98). Furthermore, a correlation existed between the percentage lymphocyte count in the BAL fluid and the ratio of macrophage to monocyte antigen-presenting capability for both PPD (r = 0.92; 95% confidence interval, 0.83 to 0.99) and SKSD (r = 0.90; 95% confidence interval, 0.45 to 0.98). In the normal subjects, AM were also unable to act effectively as accessory cells for the presentation of PPD and SKSD in the majority of subjects. No correlation existed between the percentage lymphocytes in BAL fluid and the ratio of AM to monocyte accessory cell function. These results suggest an association between AM accessory function and the presence of BAL lymphocytes in bronchial asthma.