Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.
Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.
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DOI:
10.1039/c4cc08665a
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发表时间:
2015-03-28
期刊:
影响因子:
--
通讯作者:
Ashton RS
中科院分区:
文献类型:
--
作者:
Knight GT;Sha J;Ashton RS
In situ regulation of the morphology of neural tissues derived from human pluripotent stem cells using micropatterned, clickable substrates. We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morphology and substrate biochemistry regulate tissue morphogenesis.
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