Expression level of BTN3A1 on the surface of CD14+ monocytes is a potential predictor of γδ T cell expansion efficiency
Expression level of BTN3A1 on the surface of CD14+ monocytes is a potential predictor of γδ T cell expansion efficiency
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CD14+ 单核细胞表面 BTN3A1 的表达水平是 γδ T 细胞扩增效率的潜在预测因子
DOI：
10.1016/j.bbrc.2021.12.060
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发表时间：
2022
影响因子：
3.1
通讯作者：
Ashihara Eishi
中科院分区：
文献类型：
--
作者：
Tomogane Mako;Omura Maho;Sano Yusuke;Shimizu Daiki;Toda Yuki;Hosogi Shigekuni;Kimura Shinya;Ashihara Eishi
表达 Vγ9Vδ2 T 细胞受体的人类 γδ T 细胞对病原体和恶性细胞产生强烈的反应。这些细胞由 BTN3A1 激活，BTN3A1 由病原体来源的磷酸抗原 (pAg) 或宿主来源的 pAg 表达，这些 pAg 在转化细胞或暴露于氨基二磷酸盐的细胞中积累。激活的 Vδ2 (+) T 细胞发挥多种效应功能；因此，它们是免疫疗法的有前途的候选者。然而，并非所有供体的 γδ T 细胞都具有足够的增殖活性。在这里，我们对 20 名健康捐赠者的 γδ T 细胞进行了体外培养，并探讨了可能影响其扩增效率的因素。与之前的研究一致，我们发现γδ T细胞的扩增需要CD14+单核细胞作为辅助细胞。我们还在此表明单核细胞 BTN3A1 的表面表达与 γδ T 细胞扩增呈正相关。此外，BTN3A1-Fc 处理提高了来自含有扩增能力较差的 γδ T 细胞的供体的外周血单核细胞 (PBMC) 的扩增效率。综上所述，数据表明单核细胞表面表达的 BTN3A1 水平是预测 γδ T 细胞扩增程度的有用生物标志物。
Human γδ T cells expressing Vγ9Vδ2 T cell receptors exert a robust response to pathogens and malignant cells. These cells are activated by BTN3A1, which is expressed by pathogen-derived phosphoantigens (pAgs) or host-derived pAgs that accumulate in transformed cells or in cells exposed to aminobisphosphonates. Activated Vδ2 (+) T cells exert multiple effector functions; therefore, they are a promising candidate for immunotherapy. However, not all donors have γδ T cells with adequate proliferative activity. Here, we performedex vivoculture of γδ T cells from 20 healthy donors and explored factors that may affect their expansion efficiency. Consistent with previous studies, we found that amplification of γδ T cells requires CD14+monocytes to act as accessory cells. We also show here that surface expression of BTN3A1 by monocytes correlates positively with γδ T cell expansion. Moreover, treatment with BTN3A1-Fc increased the expansion efficiency of peripheral blood mononuclear cells (PBMCs) from donors harboring γδ T cells with poor expansion capacity. Taken together, the data suggest that the level of BTN3A1 expressed on the surface of monocytes is a useful biomarker for predicting the degree of expansion of γδ T cells.