Arabidopsis plasmodesmal proteome.
Arabidopsis plasmodesmal proteome.
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DOI：
10.1371/journal.pone.0018880
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发表时间：
2011-04-20
期刊：
影响因子：
3.7
通讯作者：
Maule A
中科院分区：
文献类型：
--
作者：
Fernandez-Calvino L;Faulkner C;Walshaw J;Saalbach G;Bayer E;Benitez-Alfonso Y;Maule A
植物的多细胞特性要求细胞之间相互沟通以协调基本功能。这在一定程度上是通过细胞壁上称为胞间连丝的小孔的分子通量实现的。我们描述了从拟南芥悬浮细胞壁上纯化的胞间连丝的蛋白质组学分析。分离的胞间连丝被认为是富含膜的结构，在很大程度上缺乏质膜、内质网和细胞质成分的免疫反应性标志物。利用纳米液相色谱和Orbitrap离子阱串联质谱仪鉴定了1341个蛋白。我们把这个列表称为胞间连丝或pd -蛋白质组。相对于其他细胞壁蛋白质组，pd -蛋白质组在壁蛋白中缺失，而在膜蛋白中富集，但仍有相当数量（35%）的假定细胞质污染物，这可能反映了蛋白质组检测系统的敏感性。为了验证pd蛋白质组，我们搜索了已知的胞间连丝蛋白，并使用分子和细胞生物学技术从一小部分候选蛋白中鉴定出新的推定的胞间连丝蛋白。pd -蛋白质组包含已知的胞间连丝蛋白和一些推断的胞间连丝蛋白，基于序列或功能同源性与不同植物系统中鉴定的例子。其中许多具有膜关联，反映了孤立结构的膜性性质。利用这种联系，我们分析了大量的受体样膜蛋白样本和少量随机选择的其他膜蛋白，以观察它们作为荧光标记融合蛋白靶向间连丝的能力。从15种候选蛋白中，我们确定了3种受体样激酶、1种四联蛋白和1种功能未知的蛋白作为新的潜在的胞浆原丝蛋白。结合已发表的研究成果，这些数据表明，胞间连丝中的膜成分可能具有丰富的受体样功能，并验证了pd蛋白质组的含量为进一步揭示胞间连丝作为植物细胞间通讯关键成分的结构和功能提供了宝贵的资源。
The multicellular nature of plants requires that cells should communicate in order to coordinate essential functions. This is achieved in part by molecular flux through pores in the cell wall, called plasmodesmata. We describe the proteomic analysis of plasmodesmata purified from the walls of Arabidopsis suspension cells. Isolated plasmodesmata were seen as membrane-rich structures largely devoid of immunoreactive markers for the plasma membrane, endoplasmic reticulum and cytoplasmic components. Using nano-liquid chromatography and an Orbitrap ion-trap tandem mass spectrometer, 1341 proteins were identified. We refer to this list as the plasmodesmata- or PD-proteome. Relative to other cell wall proteomes, the PD-proteome is depleted in wall proteins and enriched for membrane proteins, but still has a significant number (35%) of putative cytoplasmic contaminants, probably reflecting the sensitivity of the proteomic detection system. To validate the PD-proteome we searched for known plasmodesmal proteins and used molecular and cell biological techniques to identify novel putative plasmodesmal proteins from a small subset of candidates. The PD-proteome contained known plasmodesmal proteins and some inferred plasmodesmal proteins, based upon sequence or functional homology with examples identified in different plant systems. Many of these had a membrane association reflecting the membranous nature of isolated structures. Exploiting this connection we analysed a sample of the abundant receptor-like class of membrane proteins and a small random selection of other membrane proteins for their ability to target plasmodesmata as fluorescently-tagged fusion proteins. From 15 candidates we identified three receptor-like kinases, a tetraspanin and a protein of unknown function as novel potential plasmodesmal proteins. Together with published work, these data suggest that the membranous elements in plasmodesmata may be rich in receptor-like functions, and they validate the content of the PD-proteome as a valuable resource for the further uncovering of the structure and function of plasmodesmata as key components in cell-to-cell communication in plants.
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DOI：
10.1073/pnas.0506958103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Dunkley, TPJ;Hester, S;Lilley, KS
通讯作者：
Lilley, KS
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Ding, XS;Liu, JZ;Nelson, RS
通讯作者：
Nelson, RS
影响因子：
3.4
作者：
Bayer, EM;Bottrill, AR;Maule, AJ
通讯作者：
Maule, AJ
DOI：
10.1073/pnas.252427999
发表时间：
2002-12-10
影响因子：
11.1
作者：
Aoki, K;Kragler, F;Lucas, WJ
通讯作者：
Lucas, WJ