A single locus leads to resistance of Arabidopsis thaliana to bacterial wilt caused by Ralstonia solanacearum through a hypersensitive-like response
A single locus leads to resistance of Arabidopsis thaliana to bacterial wilt caused by Ralstonia solanacearum through a hypersensitive-like response
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DOI：
10.1094/phyto.1999.89.8.673
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发表时间：
1999-08-01
期刊：
影响因子：
3.2
通讯作者：
Yang, CH
中科院分区：
文献类型：
--
作者：
Ho, GD;Yang, CH
青枯雷尔氏菌的菌株已被证明在一些但不是全部的拟南芥生态型中引起细菌性枯萎病。本文证明了抗病生态型S96的叶片接种R.青枯菌菌株Ps 95在接种部位周围迅速出现坏死，并且在植物中没有进一步的症状发展。感病生态型N913在接种Ps 95后7天叶片完全萎蔫，接种后2周内症状在全株系统蔓延。这些结果表明，拟南芥S96的抗病性与拟南芥的抗病性有关。青枯病是由于类似于在其他植物疾病中观察到的过敏反应（HR）的反应。北方印迹分析的防御相关基因的表达，已知在拟南芥的HR差异诱导，表明病程相关的蛋白PR-1，谷胱甘肽S-转移酶（GST 1），和铜，锌超氧化物歧化酶（SOD）的mRNA增加显着S96叶片与Ps 95浸润后3至12小时之间。Ps 95对感病生态型N913中这些基因的诱导明显延迟。抗感生态型S96与感病生态型N913杂交后代的遗传分析表明，抗Ps 95的遗传是由一个显性基因控制的。
Strains of Ralstonia solanacearum have been shown to cause bacterial wilt in some, but not all, ecotypes of Arabidopsis thaliana. We demonstrate here that after inoculation of the leaves of resistant ecotype S96 with R. solanacearum strain Ps95 necrosis around the inoculation site rapidly appeared and no further symptoms developed in the plant. Leaves of susceptible ecotype N913 completely wilted 7 days after inoculation with Ps95, and symptoms spread systemically throughout the whole plant within 2 weeks after inoculation. These results suggest that the resistance of Arabidopsis S96 to R. solanacearum is due to a response similar to the hypersensitive response (HR) observed in other plant diseases. Northern blot analysis of the expression of defense-related genes, known to be differentially induced during the HR in Arabidopsis, indicated that pathogenesis-related protein PR-1, glutathione S-transferase (GST1), and Cu,Zn superoxide dismutase (SOD) mRNAs increased significantly in S96 leaves between 3 to 12 h after infiltration with Ps95. The induction of these genes in susceptible ecotype N913 by Ps95 was clearly delayed. Genetic analysis of crosses between resistant ecotype S96 and susceptible ecotype N913 indicated that resistance to Ps95 is due to a single dominant locus.