Glucose transporter expression is developmentally regulated in in vitro derived bovine preimplantation embryos
Glucose transporter expression is developmentally regulated in in vitro derived bovine preimplantation embryos
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DOI：
10.1002/mrd.1099
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发表时间：
2001-11-01
影响因子：
2.5
通讯作者：
Fischer, B
中科院分区：
文献类型：
--
作者：
Augustin, R;Pocar, P;Fischer, B
葡萄糖很容易被不同物种的着床前胚胎吸收和利用，然而，对着床前发育过程中葡萄糖转运蛋白表达的全面分析仍然缺乏。本研究采用RT-PCR和免疫组织化学方法，研究了促性葡萄糖转运蛋白（Glut1-5和glut1 - 8）和钠依赖性葡萄糖转运蛋白（SGLT-I）在牛卵母细胞和着床前胚胎发育16 d内的表达。胚胎通过体外试管受精（IVM-IVF）产生。Glut1、Glut3、Glut8和SGLT-I在研究的所有阶段均有表达。Glut4转录本在囊胚期首次被检测到。Glut2的表达仅限于d14和d16囊胚伸长期。果糖转运体Glut5的转录始于8 /16细胞阶段。我们的研究结果表明，在牛胚胎的体外发育过程中，葡萄糖转运蛋白有不同的表达模式，表明这些异构体在牛胚胎的不同发育阶段具有特殊的功能。(C) 2001 Wiley-Liss, Inc。
Glucose is readily been taken up and utilized by preimplantation embryos from different species, However, a comprehensive analysis of the glucose transporter expression throughout preimplantation development is still missing. Here, we have investigated the expression of facilitative glucose transporters (Glut1-5 and 8) and sodium-dependent-glucose transporter (SGLT-I) in bovine oocytes and preimplantation embryos up to d16 of development, using RT-PCR and immunohistochemistry. The embryos were produced in vitro by IVM-IVF. Glut1, Glut3, Glut8, and SGLT-I were expressed in all stages studied. Glut4 transcripts were first detected at the blastocyst stage. Glut2 expression was restricted to the period of blastocyst elongation at d14 and d16. Transcription of the fructose transporter Glut5 started at the 8-/16-cell stage. Our results show a distinct expression pattern for glucose transporters during bovine embryo development in vitro indicating specialized functions for these isoforms at different developmental stages in bovine embryos. (C) 2001 Wiley-Liss, Inc.