Molecular phylogenetics of the species-rich genus Habenaria (Orchidaceae) in the New World based on nuclear and plastid DNA sequences
Molecular phylogenetics of the species-rich genus Habenaria (Orchidaceae) in the New World based on nuclear and plastid DNA sequences
复制标题
DOI：
10.1016/j.ympev.2013.01.008
复制
发表时间：
2013-04-01
影响因子：
4.1
通讯作者：
van den Berg, Cassio
中科院分区：
文献类型：
--
作者：
Batista, Joao A. N.;Borges, Karina S.;van den Berg, Cassio
玉凤花属是陆生兰花的一个大属，分布于世界的热带和亚热带地区。该属的完整性和单系性已经讨论了很多年，在同一时间或另一个，几个属已经被包括在一个广泛定义的玉凤花属或从它分离。利用核内转录间隔区（ITS）的DNA序列研究了玉凤花属新热带区成员和非洲玉凤花属选定类群的系统发育关系。区域和质体matK基因采样从玉凤花属的151个分类群来自新热带区（约。51%），以及20种玉凤花属和Bonatea来自旧大陆。贝叶斯和简约树是一致的，在所有的分析，新热带种形成了一个高度支持的组。玉凤花属在Dolichostachyae、Podandria、Diphyllae、Ceratopetalae和Bilabrellae部分的非洲种与新热带分支形成了一个高度支持的“核心玉凤花属分支”，其中包括来自新大陆的模式种。树的拓扑结构表明非洲起源的新热带区分支和低序列分歧之间的新热带区物种表明最近辐射的属在新大陆。种Bonatea和玉凤花节Chlorinae和Multipartitae形成了一个支持良好的分支，是姐妹的“核心玉凤花分支”。新热带区分支由至少21个得到充分支持的亚群组成，但目前的分区分类的所有新热带区部分都是并系或多系的，需要广泛的修订和重新界定。大多数新热带亚群形成了形态上一致的物种组合，但也有一些亚群内部形态分歧和亚群之间形态趋同的情况，表明单凭形态学可能会误导推断属内的关系。Bertauxia属，Kusibabella属和Habenella属，从新世界玉凤花属中分离出来，不是单系的，并且修改截面分类而不是属的划分似乎是最合适的。我们的研究结果不支持广泛的属碎片玉凤花如前所述，并将提供一个框架，修订属下分类和调查模式的形态进化和地理分布的属在新的世界。(C)2013 Elsevier Inc. All rights reserved.
Habenaria is a large genus of terrestrial orchids distributed throughout the tropical and subtropical regions of the world. The integrity and monophyly of this genus have been under discussion for many years, and at one time or another, several genera have been either included in a broadly defined Habenaria or segregated from it. In this study, the phylogenetic relationships of the Neotropical members of the genus and selected groups of African Habenaria were investigated using DNA sequences from the nuclear internal transcribed spacer (ITS) region and the plastid matK gene sampled from 151 taxa of Habenaria from the Neotropics (ca. 51% of the total) as well as 20 species of Habenaria and Bonatea from the Old World. Bayesian and parsimony trees were congruent with each other, and in all analyses, the Neotropical species formed a highly supported group. African species of Habenaria in sections Dolichostachyae, Podandria, Diphyllae, Ceratopetalae and Bilabrellae, and the Neotropical clade formed a highly supported "core Habenaria clade", which includes the type species of the genus from the New World. The topology of the trees indicates an African origin for the Neotropical clade and the low sequence divergence among the Neotropical species suggests a recent radiation of the genus in the New World. Species of Bonatea and Habenaria sections Chlorinae and Multipartitae formed a well-supported clade that was sister to the "core Habenaria clade". The Neotropical clade consists of at least 21 well-supported subgroups, but all Neotropical sections of the current sectional classification are paraphyletic or polyphyletic and will need extensive revision and recircumscription. Most of the Neotropical subgroups formed morphologically uniform assemblage of species, but some cases of morphological divergence within subgroups and convergence between subgroups indicated that morphology alone can be misleading for inferring relationships within the genus. The genera Bertauxia, Kusibabella and Habenella, segregated from New World Habenaria, are not monophyletic and a revision of the sectional classification rather than a generic division seems most appropriate. Our results do not support an extensive generic fragmentation of Habenaria as previously suggested and will provide a framework for revising the infrageneric classification and investigating the patterns of morphological evolution and geographical distribution of the genus in the New World. (C) 2013 Elsevier Inc. All rights reserved.