Microbubble sizing and shell characterization using flow cytometry.
Microbubble sizing and shell characterization using flow cytometry.
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DOI：
10.1109/tuffc.2011.1896
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Matula TJ
中科院分区：
文献类型：
--
作者：
Tu J;Swalwell JE;Giraud D;Cui W;Chen W;Matula TJ
Experiments were performed to size, count, and obtain shell parameters for individual ultrasound contrast microbubbles using a modified flow cytometer. Light scattering was modeled using Mie theory, and applied to calibration beads to calibrate the system. The size distribution and population were measured directly from the flow cytometer. The shell parameters (shear modulus and shear viscosity) were quantified at different acoustic pressures (from 95 to 333 kPa) by fitting microbubble response data to a bubble dynamics model. The size distribution of the contrast agent microbubbles is consistent with manufacturer specifications. The shell shear viscosity increases with increasing equilibrium microbubble size, and decreases with increasing shear rate. The observed trends are independent of driving pressure amplitude. The shell elasticity does not vary with microbubble size. The results suggest that a modified flow cytometer can be an effective tool to characterize the physical properties of microbubbles, including size distribution, population, and shell parameters.
