Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain.
Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain.
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DOI：
10.1186/1743-422x-6-58
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发表时间：
2009-05-14
期刊：
影响因子：
4.8
通讯作者：
Johne R
中科院分区：
文献类型：
--
作者：
Schielke A;Sachs K;Lierz M;Appel B;Jansen A;Johne R
戊型肝炎是中欧越来越多的人类疾病。除了戊型肝炎病毒（HEV）感染高度流行的家猪外，野猪也被确定为人类感染的可能来源。为了评估戊型肝炎病毒在德国野猪种群中的分布，我们检测了来自不同地理区域的肝脏样本中戊型肝炎病毒基因组的存在，并将检测到的序列与动物和人戊型肝炎病毒株进行了比较。共检测了148份野猪肝脏样品，采用实时荧光定量PCR方法检测，平均HEV检出率为14.9%（95%CI 9.6-21.6）。在所有年龄组和所有地理区域均检测到戊型肝炎病毒。然而，戊型肝炎病毒感染的患病率在农村地区明显高于城市地区（p < 0.001）。PCR产物的测序结果表明，基因型3内检测到的病毒具有高度的异质性，并根据其地理来源进行分组。测定了在联邦勃兰登堡州的许多野猪中检测到的戊型肝炎病毒分离株（wbGER 27）的全基因组序列。它属于基因型3 i，与来自德国的人戊型肝炎患者的部分序列显示97.9%的核苷酸序列同一性。结果表明，野猪被认为是一个水库的戊型肝炎病毒在德国和戊型肝炎病毒传播给人类的风险是存在于农村以及城市地区。
Hepatitis E is an increasingly diagnosed human disease in Central Europe. Besides domestic pigs, in which hepatitis E virus (HEV) infection is highly prevalent, wild boars have been identified as a possible source of human infection. In order to assess the distribution of HEV in the wild boar population of Germany, we tested liver samples originating from different geographical regions for the presence of the HEV genome and compared the detected sequences to animal and human HEV strains. A total of 148 wild boar liver samples were tested using real-time RT-PCR resulting in an average HEV detection rate of 14.9% (95% CI 9.6–21.6). HEV was detected in all age classes and all geographical regions. However, the prevalence of HEV infection was significantly higher in rural as compared to urban regions (p < 0.001). Sequencing of the PCR products indicated a high degree of heterogenicity of the detected viruses within genotype 3 and a grouping according to their geographical origin. The whole genome sequence of an HEV isolate (wbGER27) detected in many wild boars in the federal state of Brandenburg was determined. It belongs to genotype 3i and shows 97.9% nucleotide sequence identity to a partial sequence derived from a human hepatitis E patient from Germany. The results indicate that wild boars have to be considered as a reservoir for HEV in Germany and that a risk of HEV transmission to humans is present in rural as well as urban regions.