CHARACTERIZATION OF ANGUIBACTIN, A NOVEL SIDEROPHORE FROM VIBRIO-ANGUILLARUM 775(PJM1)
CHARACTERIZATION OF ANGUIBACTIN, A NOVEL SIDEROPHORE FROM VIBRIO-ANGUILLARUM 775(PJM1)
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DOI：
10.1128/jb.167.1.57-65.1986
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发表时间：
1986-07-01
影响因子：
3.2
通讯作者：
SANDERSLOEHR, J
中科院分区：
文献类型：
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作者：
ACTIS, LA;FISH, W;SANDERSLOEHR, J
从缺铁培养物的上清液中分离出了由鳗弧菌775携带pJM 1质粒产生的铁载体安吉菌素。通过吸附到XAD-7树脂中并随后在Sephadex LH-20柱上进行凝胶过滤来纯化该铁反应性物质。得到的中性化合物在质谱中产生m/z 348的离子，并且通过元素分析测定含有一个硫原子、四个氧原子和四个氮原子。其强紫外吸收和蓝色荧光暗示酚部分。在比色反应中，anguibactin表现得像邻苯二酚。在铁络合物中在510 nm处出现新的吸收带，以及在共振拉曼光谱中在1，367、1，447、1，469和1，538 cm-1处出现峰，支持了邻苯二酚的归属。此外，红外光谱给出了仲酰胺官能团的证据，但没有观察到游离羧酸或异羟肟酸基团。第三个铁连接组建议在铁结合过程中释放三个质子;所得材料的质谱分析产生了铁anguibactin的1：1复合物的分子离子特征。纯化的anguibactin在铁限制条件下对鳗弧菌775（pJM 1）铁载体缺陷突变体表现出特异性生长促进活性。在鳗弧菌生物测定中，大量已知的铁载体和合成螯合剂未能产生类似类型的特异性交叉摄食，这表明鳗菌素具有新的结构。
Anguibactin, a siderophore produced by cells of Vibrio anguillarum 775 harboring the pJM1 plasmid, has now been isolated from the supernatants of iron-deficient cultures. This iron-reactive material was purified by adsorption into an XAD-7 resin and subsequent gel filtration on a Sephadex LH-20 column. The resulting neutral compound produced an ion at m/z 348 in mass spectrometry and contained one sulfur, four oxygen, and four nitrogen atoms as determined by elemental analysis. Its strong UV absorbance and blue fluorescence were suggestive of a phenolic moiety. In colorimetric reactions anguibactin behaved like a catechol. The catechol assignment was supported by the appearance of a new absorption band at 510 nm in the ferric complex and by the appearance of peaks at 1,367, 1,447, 1,469, and 1,538 cm-1 in the resonance Raman spectrum. In addition, the infrared specturm gave evidence of a secndary amide function, but no free carboxylic acid or hydroxamic acid groups were observed. A third iron-ligating group was suggested by the liberation of three protons during iron binding; mass spectrometry of the resulting material yielded a molecular ion characteristic of a 1:1 complex of ferric anguibactin. The purified anguibactin exhibited specific growth-promoting activity under iron-limiting conditions for a siderophore-deficient mutant of V. anguillarum 775(pJM1). A novel structure for anguibactin was indicated by the failure of a large number of known siderophores and synthetic chelators to yield a similar type of specific cross-feeding in the V. anguillarum bioassay.