USE OF FLUORESCEIN-CONJUGATED BANDEIRAEA-SIMPLICIFOLIA B4-ISOLECTIN AS A HISTOCHEMICAL REAGENT FOR THE DETECTION OF ALPHA-D-GALACTOPYRANOSYL GROUPS - THEIR OCCURRENCE IN BASEMENT-MEMBRANES
USE OF FLUORESCEIN-CONJUGATED BANDEIRAEA-SIMPLICIFOLIA B4-ISOLECTIN AS A HISTOCHEMICAL REAGENT FOR THE DETECTION OF ALPHA-D-GALACTOPYRANOSYL GROUPS - THEIR OCCURRENCE IN BASEMENT-MEMBRANES
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DOI：
10.1016/0014-4827(79)90392-6
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发表时间：
1979-01-01
影响因子：
3.7
通讯作者：
GOLDSTEIN, IJ
中科院分区：
文献类型：
--
作者：
PETERS, BP;GOLDSTEIN, IJ
The B. simplicifolia B4-isolectin, which combines specifically with .alpha.-D-galactopyranosyl groups, was conjugated with fluorescein isothiocysnate and demonstrated to be a reliable histochemical probe for the detection of these groups in normal tissues of mouse, rabbit, rat and man. Specificity of binding of the fluorescein-conjugated B. simplicifolia B4-isolectin to native cryostat tissue sections was demonstrated in 2 ways. The hapten inhibitor methyl .alpha.-D-galactopyranoside prevented the binding of the lectin to tissues whereas the non-hapten methyl .alpha.-D-glucopyranoside did not. Pretreatment of tissue sections with coffee bean .alpha.-galactoside abolished lectin binding whereas pretreatment with Aspergillus niger or Escherichia coli .beta.-galactosidase did not. The fluorescein-conjugated isolectin visualized .alpha.-D-galactopyranosyl groups in basement membranes and on the surface of certain epithelial cells of mouse, rat, rabbit, and on the surface of the TA3 murine mammary carcinoma. These studies suggest that the B. simplicifolia B4-isolectin may be of great utility in studying the family of .alpha.-D-galactosyl-containing glycoconjugates of basement membranes in pathological states accompanied by basement membrane changes, e.g., diabetes mellitus, and in neoplasms that secrete basement membrane.
