Reconsideration of S-genotype assignments, and discovery of a new allele based on S-RNase PCR-RFLPs in Japanese pear cultivars
Reconsideration of S-genotype assignments, and discovery of a new allele based on S-RNase PCR-RFLPs in Japanese pear cultivars
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DOI：
10.1270/jsbbs.51.5
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发表时间：
2001-03-01
期刊：
影响因子：
2.4
通讯作者：
Nakanishi, T
中科院分区：
文献类型：
--
作者：
Castillo, C;Takasaki, T;Nakanishi, T
日本梨品种‘赤穗’、‘丹泽’、‘君川’、‘朝珠’、‘一原濑’和‘明月’的S基因型分配，近来引起了一些质疑。对这些品种进行了基于S-RNase的PCR-RFLP分析，并对它们的S基因型进行了重新分配。基于PCR-RFLP系统的分配是一致的，以前确定的授粉试验中确定的6个品种，'Chojuro'，'Doitsu'，'Kikusui'，'Kosui'，'Taihaku'和'Yakumo'，证实了该系统的适用性。对“赤穗”、“田泽”、“Kimizukawase”和“Choju”进行了分析，并分别指定为S3 S5、S4 S5、S1 S5和S1 S5。其中两个品种‘赤穗’和‘田泽’也通过我们的授粉试验得到了证实。（'Nijisseiki' x 'Ichiharawase'），扩增出一个新的S-RNase片段，其大小和消化模式与日本梨的S-1至S-7 RNase不同，该S-RNase高变区的氨基酸序列与S-1至S-7-RNase的氨基酸序列有很大的不同，因此我们将该S-RNase命名为S-8-RNase，并鉴定了“一原濑”的S-基因型。“Meigetsu”和“Heiwa”分别为S1 S8、S1 S8和S4 S8。
The S-genotype assignments in the Japanese pear cultivars, 'Akaho', 'Tanzawa', 'Kimizukawase', 'Choju', 'Ichiharawase' and 'Meigetsu' previously determined by pollination tests have raised some doubt recently. These cultivars mere analyzed by S-RNase based PCR-RFLP, and their S-genotype assignments reconsidered. The assignment based on the PCR-RFLP system was in agreement with that determined previously by pollination tests in six cultivars, 'Chojuro', 'Doitsu', 'Kikusui', 'Kosui', 'Taihaku' and 'Yakumo', confirming the applicability of the system. 'Akaho', 'Tanzawa', 'Kimizukawase' and 'Choju' were analyzed and assigned as S3S5, S4S5, S1S5 and S1S5, respectively. Two of these cultivars, 'Akaho' and 'Tanzawa', were also confirmed by our pollination tests, In three cultivars, 'Ichiharawase', 'Meigetsu' and 'Heiwa' ('Nijisseiki' x 'Ichiharawase'), a new S-RNase fragment with a size and digestion pattern distinct from those of S-1 to S-7 RNases of Japanese pear was amplified, The deduced amino acid sequence in the hypervariable region of this S-RNase was quite different from those of S-1 to S-7-RNases, We, therefore, assigned this S-RNase as S-8-RNase, and identified the S-genotypes of 'Ichiharawase', 'Meigetsu' and 'Heiwa' as S1S8, S1S8 and S4S8, respectively.