Two types of chloride channel on duct cells cultured from human fetal pancreas.
Two types of chloride channel on duct cells cultured from human fetal pancreas.
复制标题
从人胎儿胰腺培养的导管细胞上有两种类型的氯离子通道。
DOI：
--
复制
发表时间：
1989
影响因子：
--
通讯作者：
B. Argent
中科院分区：
文献类型：
--
作者：
M. Gray;A. Harris;L. Coleman;J. R. Greenwell;B. Argent
应用膜片钳技术，我们在培养的人胚胰腺导管细胞上发现了两种类型的氯通道。我们观察到的通道最常表现出轻微的向外整流，在细胞附着的补丁的电导为4-7 pS，并存在于顶端质膜上，它通常发生在集群。其开放状态的概率并不明显的电压依赖性，但增加了三倍，通过暴露导管细胞分泌素（10纳米），二丁酰环AMP（1毫米），或毛喉素（1 μ M）。另一种类型的氯离子通道很少观察到。其电流-电压关系表现出明显的外向整流，内向电流的弦电导为19 pS，外向电流的弦电导为53 pS。虽然这个通道可以被激活的持续去极化切除的补丁，一旦激活的开放状态的概率是不依赖于电压。虽然这些通道的生理作用尚未明确，但小电导通道可能与Cl- -HCO-3交换器平行发挥作用，为导管细胞的产电碳酸氢盐分泌提供机制。
Using the patch-clamp technique, we have identified two types of chloride channel on duct cells cultured from human fetal pancreas. The channel we observed most frequently exhibited slight outward rectification, had a conductance of 4-7 pS in cell-attached patches, and was present on the apical plasma membrane where it usually occurred in clusters. Its open-state probability was not markedly voltage dependent but was increased up to threefold by exposing duct cells to secretin (10 nM), dibutyryl cyclic AMP (1 mM), or forskolin (1 microM). The other type of chloride channel was only rarely observed. Its current-voltage relationship exhibited marked outward rectification, giving chord conductances of 19 pS for inward currents and 53 pS for outward currents. Although this channel could be activated by sustained depolarization of excised patches, once activated the open state probability was not voltage dependent. While the physiological role of these channels is not firmly established, the small-conductance channel might function in parallel with a Cl- -HCO-3 exchanger to provide a mechanism for electrogenic bicarbonate secretion from the duct cell.
DOI：
10.1126/science.2424085
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Welsh,MJ
通讯作者：
Welsh,MJ
来自犬气管上皮细胞的单氯离子通道电流。
DOI：
10.1016/0005-2736(86)90328-7
发表时间：
1986
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Shoemaker,RL;Frizzell,RA;Dwyer,TM;Farley,JM
通讯作者：
Farley,JM