HUMAN MAST-CELL HETEROGENEITY - HISTAMINE-RELEASE FROM MAST-CELLS DISPERSED FROM SKIN, LUNG, ADENOIDS, TONSILS, AND COLON IN RESPONSE TO IGE-DEPENDENT AND NONIMMUNOLOGIC STIMULI
HUMAN MAST-CELL HETEROGENEITY - HISTAMINE-RELEASE FROM MAST-CELLS DISPERSED FROM SKIN, LUNG, ADENOIDS, TONSILS, AND COLON IN RESPONSE TO IGE-DEPENDENT AND NONIMMUNOLOGIC STIMULI
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DOI：
10.1016/0091-6749(88)90199-6
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发表时间：
1988-03-01
影响因子：
14.2
通讯作者：
CHURCH, MK
中科院分区：
文献类型：
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作者：
LOWMAN, MA;REES, PH;CHURCH, MK
我们比较了抗 IgE、钙离子载体 A23187、P 物质、化合物 48/80、聚 L-赖氨酸和吗啡从人皮肤肺、腺样体、扁桃体和结肠的肥大细胞中释放组胺的能力。对所有组织使用单一胶原酶/透明质酸酶分散技术可以在不存在方法学差异的情况下进行反应性比较。所有检查组织的肥大细胞都会分泌组胺以响应抗 IgE 和钙离子载体 A23187。 然而，只有皮肤肥大细胞对 P 物质、化合物 48/80、聚-L-赖氨酸和吗啡有反应。这些非免疫刺激对人类皮肤肥大细胞的激活明显地将其与人类肺、腺样体、扁桃体和结肠的肥大细胞区分开来，并且表明人类肥大细胞群内的功能异质性。我们提出，皮肤肥大细胞上存在神经肽和碱性化合物的功能性受体位点，而粘膜或淋巴来源的肥大细胞群中不存在这些功能性受体位点，这反映了这些细胞在血管稳态中的特殊作用。
We have compared the ability of anti-IgE, calcium ionophore A23187, substance P, compound 48/80, poly-L-lysine, and morphine to release histamine from mast cells of human skin lung, adenoids, tonsils, and colon. Use of a single collagenase/hyaluronidase dispersion technique for all tissues has allowed comparisons of reactivity to be made that are free from methodological variations. Mast cells from all tissues examined secreted histamine in response to anti-IgE and calcium ionophore A23187. However, only skin mast cells were responsive to substance P, compound 48/80, poly-L-lysine, and morphine. Activation of human skin mast cells by these nonimmunologic stimuli clearly distinguishes them from the mast cells of human lung, adenoids, tonsils, and colon and is indicative of functional heterogeneity within the human mast cell population. We propose that the presence of functional receptor site for neuropeptides and basic compounds on skin mast cells that are not present in mast cell populations from mucosal or lymphoid sources reflects a specialized role for these cells in vascular homeostasis.