Structure of the molecular bushing of the bacterial flagellar motor.
Structure of the molecular bushing of the bacterial flagellar motor.
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细菌鞭毛马达的分子套管的结构。
DOI：
10.1101/2020.11.12.379347
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Yamaguchi;T.;Makino;F.;Miyata;T.;Minamino;T.;Kato;T. & Namba;K.
细菌鞭毛的基体是一个旋转的马达，由几个环(C、MS和LP)和一个杆组成。LP环起到衬套的作用，支撑远端杆快速稳定地旋转，没有太多摩擦。在这里，我们使用电子冷冻显微镜来描述来自鼠伤寒沙门氏菌的3.5 ä分辨率的杆子周围的LP环结构。该结构显示出26倍的旋转对称性和每个亚基与多达6个伙伴的错综复杂的亚基间相互作用，这解释了结构的稳定性。内表面既带正电又带负电。P环上的正电荷(嵌入在肽聚糖层中的LP环的一部分)可能在它最初围绕带负电表面的棒进行组装时起着重要作用。
The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure around the rod, at 3.5 Å resolution, fromSalmonellaTyphimurium. The structure shows 26-fold rotational symmetry and intricate intersubunit interactions of each subunit with up to six partners, which explains the structural stability. The inner surface is charged both positively and negatively. Positive charges on the P ring (the part of the LP ring that is embedded within the peptidoglycan layer) presumably play important roles in its initial assembly around the rod with a negatively charged surface.