Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks
Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks
复制标题
藏羊和牦牛肠细胞虫的基因型
DOI：
10.1007/s00436-017-5742-1
复制
发表时间：
2018-03-01
影响因子：
2
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
--
作者：
Zhang, Qiang;Cai, Jinzhong;Xiao, Lihua
对于生活在户外、高海拔极端气候条件下的藏羊和牦牛，比氏肠细胞虫基因型的分布研究还很少。在这项研究中，收集了中国青海 312 只藏羊和 554 头牦牛的粪便样本，并通过核糖体内转录间隔区的 PCR 序列分析检查了 E. bieneusi 的情况。其中，藏羊标本73份（23.4%）、牦牛标本40份（7.2%）检出比氏艾美耳球虫阳性。藏羊中存在8个E. bieneusi基因型，其中3个已知基因型（BEB6、COS-I和NESH5）和5个新基因型（命名为CHS13-CHS17）。同样，在牦牛中发现了 7 种 E. bieneusi 基因型，其中包括 5 种已知基因型（J、BEB4、BEB6、COS-I 和 NESH5）和 2 种新基因型（命名为 CHN13 和 CHN14）。研究中发现的大多数 E. bieneusi 基因型和所有常见基因型都属于第 2 组。第 1 组中发现了一个新的基因型亚组。 E. bieneusi 基因型在藏羊和牦牛中的分布不同，其中 BEB6 为藏羊中的优势基因型（42.5%），J 为牦牛中的优势基因型（47.5%）。这些数据支持第 2 组内 E. bieneusi 基因型之间发生宿主适应。
Few studies have been conducted on the distribution of Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks, which live outdoors in extreme climate with high altitude. In this study, fecal specimens from 312 Tibetan sheep and 554 yaks in Qinghai, China, were collected and examined for E. bieneusi by PCR-sequence analysis of the ribosomal internal transcribed spacer. Among them, 73 (23.4%) specimens from Tibetan sheep and 40 (7.2%) from yaks were positive for E. bieneusi. There were eight E. bieneusi genotypes in Tibetan sheep, including three known ones (BEB6, COS-I, and NESH5) and five novel ones (named as CHS13-CHS17). Similarly, seven E. bieneusi genotypes were found in yaks, including five known ones (J, BEB4, BEB6, COS-I, and NESH5) and two novel ones (named as CHN13 and CHN14). Most of the E. bieneusi genotypes and all frequent ones identified in the study belonged to group 2. One new subgroup of genotypes was identified within group 1. The distribution of E. bieneusi genotypes was different between Tibetan sheep and yaks, with BEB6 as the dominant one (42.5%) in Tibetan sheep and J as the dominant one (47.5%) in yaks. These data support the occurrence of host adaptation among E. bieneusi genotypes within group 2.