PSEUDOMONAS-OLEOVORANS AS A SOURCE OF POLY(BETA-HYDROXYALKANOATES) FOR POTENTIAL APPLICATIONS AS BIODEGRADABLE POLYESTERS
PSEUDOMONAS-OLEOVORANS AS A SOURCE OF POLY(BETA-HYDROXYALKANOATES) FOR POTENTIAL APPLICATIONS AS BIODEGRADABLE POLYESTERS
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DOI：
10.1128/aem.54.8.1977-1982.1988
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发表时间：
1988-08-01
影响因子：
4.4
通讯作者：
FULLER, RC
中科院分区：
文献类型：
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作者：
BRANDL, H;GROSS, RA;FULLER, RC
油橄榄假单胞菌生长在含有正烷酸的均匀培养基中，从甲酸到癸酸，作为唯一的碳源。细胞内多聚体的形成。-羟基烷酸酯)只观察到己酸酯和更高的n-烷酸酯。无论在辛酸盐还是壬酸盐上生长，最大分离的聚合物产率约为细胞干重的30%。在大多数情况下，聚合物中的主要重复单元与用于生长的正烷酸具有相同的链长，但与用作碳源的酸相比，具有两个碳原子的单元也通常存在于形成的聚酯中。事实上，共聚物含有多达六种不同类型的。-羟基烷酸酯单位。聚（. β）的平均分子量。P. oleovorans生产的-羟基烷酸酯)共聚物从90,000到370,000不等。尽管辛酸盐和壬酸盐的细胞产量更高，但己酸盐和庚酸盐的使用产生了更高分子量的聚合物。这些共聚酯代表了一类全新的可生物降解热塑性塑料。
Pseudomonas oleovorans was grown in homogeneous media containing n-alkanoic acids, from formate to decanoate, as the sole carbon sources. Formation of intracellular poly(.beta.-hydroxyalkanoates) was observed only for hexanoate and the higher n-alkanoic acids. The maximum isolated polymer yields were approximately 30% of the cellular dry weight with growth on either octanoate or nonanoate. In most cases, the major repeating unit in the polymer had the same chain length as the n-alkanoic acid used for growth, but units with two carbon atoms less or more than the acid used as a carbon source were also generally present in the polyesters formed. Indeed, copolymers containing as many as six different types of .beta.-hydroxyalkanoate units were performed. The weight average molecular weights of the poly(.beta.-hydroxyalkanoate) copolymers produced by P. oleovorans ranged from 90,000 to 370,000. In spite of the higher cell yields obtained with octanoate and nonanoate, the use of hexanoate and heptanoate yielded higher-molecular-weight polymers. These copolyesters represent an entirely new class of biodegradable thermoplastics.