G protein mRNA mapped in rat brain by in situ hybridization.
G protein mRNA mapped in rat brain by in situ hybridization.
复制标题
通过原位杂交绘制大鼠大脑中 G 蛋白 mRNA 的图谱。
DOI：
10.1073/pnas.85.8.2864
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发表时间：
1988
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Largent,BL;Jones,DT;Reed,RR;Pearson,RC;Snyder,SH
鸟嘌呤核苷酸结合调节蛋白（G 蛋白）介导许多受体偶联信号转导事件。我们通过原位杂交在大鼠大脑中定位了 G 蛋白亚基——G α s、G α o 和 G β 的 mRNA。通过一种技术对寡核苷酸探针进行放射性标记，从而产生具有确定的比活性和统一长度的探针。编码 G α 和 G β 的 mRNA 种类以高密度异质性存在于整个大脑中，尤其是在大的神经元细胞体中，例如海马锥体细胞、齿状回的颗粒细胞、下丘脑核以及脑干核和网状结构的神经元。 G α o mRNA 的分布和丰度更为有限，可在屏状体、内核状核、缰核、海马锥体细胞、齿状回颗粒细胞和小脑浦肯野细胞中检测到。
Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits--G alpha s, G alpha o, and G beta. Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G alpha s and G beta occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies--e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G alpha o mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.