Living materials with programmable functionalities grown from engineered microbial co-cultures
Living materials with programmable functionalities grown from engineered microbial co-cultures
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DOI：
10.1038/s41563-020-00857-5
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发表时间：
2021-01-11
期刊：
影响因子：
41.2
通讯作者：
Ellis, Tom
中科院分区：
文献类型：
--
作者：
Gilbert, Charlie;Tang, Tzu-Chieh;Ellis, Tom
生物系统组装具有自主模式的生物材料，这些材料能够自我修复，并且能够感知并响应其环境。工程生物材料领域旨在利用基因工程生物创造具有类似于天然生物材料性能的新型材料。在此，我们描述了一种利用酿酒酵母（Saccharomyces cerevisiae）和产细菌纤维素的鼠李糖乳杆菌（Komagataeibacter rhaeticus）的稳定共培养来制造基于功能性细菌纤维素的生物材料的方法。可以对酵母菌株进行工程改造，使其向细菌纤维素中分泌酶，从而产生自主生长的催化材料，并能够对细菌纤维素的整体性质进行DNA编码修饰。或者，可以将工程酵母掺入正在生长的纤维素基质中，创造出能够感知并响应化学和光学刺激的生物材料。这种细菌和酵母的共生培养是一个灵活的平台，用于生产基于细菌纤维素的工程生物材料，在生物传感和生物催化方面具有潜在应用。细菌和酵母的共生培养被用于制造基于细菌纤维素的生物材料，这些材料能够响应外部信号并调整其结构和功能特性，对传感和催化应用具有重要意义。
Biological systems assemble living materials that are autonomously patterned, can self-repair and can sense and respond to their environment. The field of engineered living materials aims to create novel materials with properties similar to those of natural biomaterials using genetically engineered organisms. Here, we describe an approach to fabricating functional bacterial cellulose-based living materials using a stable co-culture of Saccharomyces cerevisiae yeast and bacterial cellulose-producing Komagataeibacter rhaeticus bacteria. Yeast strains can be engineered to secrete enzymes into bacterial cellulose, generating autonomously grown catalytic materials and enabling DNA-encoded modification of bacterial cellulose bulk properties. Alternatively, engineered yeast can be incorporated within the growing cellulose matrix, creating living materials that can sense and respond to chemical and optical stimuli. This symbiotic culture of bacteria and yeast is a flexible platform for the production of bacterial cellulose-based engineered living materials with potential applications in biosensing and biocatalysis.A symbiotic culture of bacteria and yeast is used to fabricate bacterial cellulose-based living materials that respond to external cues and adapt their structural and functional properties, with implications for sensing and catalytic applications.